Yue Hao, Pi Zi-feng, Song Feng-rui, Liu Zhi-qiang, Liu Shu-ying
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun 130022, China.
Yao Xue Xue Bao. 2007 Feb;42(2):201-5.
The method was established study the influence of different herbal combination with Radix Aconiti in the traditional medical formulae on content of the aconite alkaloids, for elucidating the scientific basis of reducing the toxicity of aconite in traditional Chinese medical formulation. The samples for ESI-MS study were prepared by decocting a mixture of Radix Aconiti Lateralis Preparata ( RALP) with Radix Glycyrrhizae Preparata (RGP) , Radix Paeoniae Alba ( RPA) , Rhizoma Zingiberis (RZ) or Radix Et Rhizoma Rhei ( RERR) , separately, and extracting the residue of the above mentioned mixtures after decocting. The diester-diterpenoid alkaloids (DDAs) was lower in the herb couples of RALP-RGP, RALP-RPA, RALP-RZ and RALP-RERR, and lipo-alkaloids was increased in the herb couples of RALP-RGP, RALP-RPA and RALP-RZ. The reason of reducing toxic effect principle is that the components of RGP, RPA and RZ have ester-exchange reactions with DDAs in RALP to produce lipo-alkaloids of low toxicity in the decocting process of the herb couples. The combination of RALP-RERR can reduce the content of DDAs in decoction and residue due to the formation of water insoluble alkaloid compound.
该方法旨在研究传统方剂中不同与附子配伍的药材对附子生物碱含量的影响，以阐明中药方剂中降低附子毒性的科学依据。用于电喷雾电离质谱（ESI-MS）研究的样品，通过分别将制附子（RALP）与炙甘草（RGP）、白芍（RPA）、干姜（RZ）或大黄（RERR）混合煎煮，并提取上述混合物煎煮后的残渣来制备。在RALP-RGP、RALP-RPA、RALP-RZ和RALP-RERR药对中，双酯二萜生物碱（DDAs）含量降低，而在RALP-RGP、RALP-RPA和RALP-RZ药对中脂溶性生物碱含量增加。降低毒性作用的原理是，在药对煎煮过程中，RGP、RPA和RZ的成分与RALP中的DDAs发生酯交换反应，生成低毒的脂溶性生物碱。RALP-RERR组合可使煎出液和残渣中DDAs含量降低，这是由于形成了水不溶性生物碱化合物。
