Wang Yong, Liu Zhi-qiang, Song Feng-rui, Liu Shu-ying
Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Department of Chemistry, Northeast Normal University, Changchun, China.
Yao Xue Xue Bao. 2003 Jun;38(6):451-4.
To explore the combination principle about aconite roots from the chemical point of view.
Analyzing and comparing the nordeterpenoid alkaloids in the crude aconite roots, the decoction of crude aconite roots and aconite roots with some other Chinese traditional herbs by electrospray ionization trap tandem mass spectrometry. Detecting the pH value of the decoction of crude aconite roots and of some other Chinese traditional herbs; studying the influence of acidity to the hydrolysis of standard sample of mesaconitine.
Most of diester-diterpenoid alkaloids in the decoction of aconite roots and the decoction of aconite roots with liquorice roots are hydrolyzed, and the hydrolysis of alkaloids are inhibited in the decoction of aconite roots with Pinellia tuber or Schisandra. The acidity of the decoction of aconite roots or liquorice is lower than the decoction of Pinellia tuber or Schisandra; standard sample of mesaconitine can not be hydrolyzed in acid water.
Acidity is a key factor to influence the hydrolysis of diester-diterpenoid alkaloids, and the hydrolysis of alkaloids are inhibited in high acidity, which lead to the high toxicity of the decoction containing aconite roots.
从化学角度探讨附子的配伍原理。
采用电喷雾电离阱串联质谱法分析比较附子生药、附子水煎液以及附子与其他中药配伍后的二萜生物碱成分。检测附子生药及其他中药水煎液的pH值；研究酸度对中乌头碱标准品水解的影响。
附子水煎液及附子与甘草配伍水煎液中多数双酯型二萜生物碱发生水解，而附子与半夏或五味子配伍水煎液中生物碱水解受到抑制。附子或甘草水煎液的酸度低于半夏或五味子水煎液；中乌头碱标准品在酸性水中不发生水解。
酸度是影响双酯型二萜生物碱水解的关键因素，高酸度下生物碱水解受到抑制，这导致含附子水煎液毒性较高。
