Tazumi A, Saito S, Sekizuka T, Murayama O, Moore J E, Millar B C, Matsuda M
Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe, Sagamihara, Japan.
J Basic Microbiol. 2007 Jun;47(3):260-5. doi: 10.1002/jobm.200610256.
The 3,339 base pair (bp) sequences encoding a putative open reading frame (ORF), non-coding promoter and leader regions (approximately 320 bp), full-length 16S ribosomal RNA (rRNA) gene (approximate 1,540 bp) and part of the 16S-23S rDNA internal spacer region (ISR) were determined from genome DNA libraries of the Taylorella asinigenitalis (UK-1) isolate. The non-coding promoter and leader regions included antiterminators (boxB, boxA and boxC) immediately upstream of the 16S rRNA gene sequence. An approximately 680 bp region upstream of the non-coding promoter region appears to contain a putative ORF with high sequence similarity to GTP cyclohydrolase I. In addition, a typical order of intercistronic tRNA genes with the 48 nucleotide spacer of 5'-16S rDNA-tRNA(Ile)-tRNA(Ala)-23S rDNA-3' was demonstrated in a part of the 16S-23S rDNA ISR. The antiterminators of boxB and boxA were also identified in the ISR.A phylogenetic analysis based on the 16S rRNA gene sequence information clearly demonstrated that the five T. asinigenitalis isolates formed a cluster together with the three T. equigenitalis strains, more similar to Pelistega europaea than the other beta-Proteobacteria from the 13 species of 11 genera.
从马泰勒菌（UK-1）分离株的基因组DNA文库中确定了编码假定开放阅读框（ORF）、非编码启动子和前导区（约320 bp）、全长16S核糖体RNA（rRNA）基因（约1540 bp）以及16S-23S rDNA内部间隔区（ISR）部分的3339个碱基对（bp）序列。非编码启动子和前导区包括16S rRNA基因序列上游紧邻的抗终止子（boxB、boxA和boxC）。非编码启动子区域上游约680 bp的区域似乎包含一个与GTP环化水解酶I具有高度序列相似性的假定ORF。此外，在16S-23S rDNA ISR的一部分中证实了具有典型顺序的顺反子间tRNA基因，其间隔为48个核苷酸，顺序为5'-16S rDNA-tRNA（Ile）-tRNA（Ala）-23S rDNA-3'。在ISR中也鉴定出了boxB和boxA的抗终止子。基于16S rRNA基因序列信息的系统发育分析清楚地表明，五株马泰勒菌分离株与三株马生殖道泰勒菌菌株一起形成了一个聚类，与欧洲佩利斯特菌的相似性高于来自11个属13个物种的其他β-变形菌。
