Tazumi Akihiro, Saito Satoru, Sekizuka Tsuyoshi, Murayama Ohoshi, Takamiya Shinzaburo, Moore John E, Millar B Cherie, Matsuda Motoo
Laboratory of Molecular Biology, School of Environmental Health Sciences, Azabu University, Fuchinobe 1-17-71, Sagamihara 229-8501, Japan.
Antonie Van Leeuwenhoek. 2007 Aug;92(2):257-64. doi: 10.1007/s10482-007-9144-8. Epub 2007 Mar 14.
An approximately 4.2 kbp region encoding 23S and 5S rRNA genes was identified when recombinant plasmid DNAs from two genomic DNA libraries and an inverse PCR product of Taylorella asinigenitalis UK-1 isolate were analyzed. Full-length genes of 23S rRNA (3,225 bp) and 5S rRNA (117 bp) of T. asinigenitalis are described. The present sequence analysis identified a non-coding hypothetically intrinsic transcription terminator region downstream of the 5S rRNA gene. The sequence, however, downstream of the 5S rRNA gene did not show any distal tRNA genes. Surprisingly, an intervening sequence (IVS) of 270 bp in length, including two specific tandem repeat units of 80 bp and one partial unit of 48 bp with unknown functions was identified in the first quarter of the 23S rRNA gene sequence. A second IVS of 70 bp in length was also identified in the central region of the 23S rRNA gene. In addition, by using PCR and sequencing procedures, two T. asinigenitalis isolates, UK-1 and UK-2, carried multiple IVSs in the first quarter and central regions. Moreover, the 23S rRNA fragmentation occurred in the UK-1 isolate. A phylogenetic analysis was first carried out based on the 23S rRNA sequence data from T. asinigenitalis UK-1 and 13 other beta-Proteobacteria. This is the first report of IVSs in the 23S rRNA gene from the beta-Proteobacteria.
当对来自两个基因组DNA文库的重组质粒DNA以及亚洲泰勒菌UK-1分离株的反向PCR产物进行分析时，鉴定出了一个约4.2 kbp的区域，该区域编码23S和5S rRNA基因。描述了亚洲泰勒菌23S rRNA（3225 bp）和5S rRNA（117 bp）的全长基因。目前的序列分析在5S rRNA基因下游鉴定出一个非编码的假设性固有转录终止子区域。然而，5S rRNA基因下游的序列未显示任何远端tRNA基因。令人惊讶的是，在23S rRNA基因序列的第一季度中鉴定出一个长度为270 bp的间隔序列（IVS），包括两个80 bp的特定串联重复单元和一个功能未知的48 bp部分单元。在23S rRNA基因的中央区域还鉴定出了一个长度为70 bp的第二个IVS。此外，通过PCR和测序程序，亚洲泰勒菌的两个分离株UK-1和UK-2在第一季度和中央区域携带多个IVS。此外，UK-1分离株中发生了23S rRNA片段化。首先基于亚洲泰勒菌UK-1和其他13种β-变形菌的23S rRNA序列数据进行了系统发育分析。这是β-变形菌23S rRNA基因中IVS的首次报道。
