Hosseinkhani Hossein, Hosseinkhani Mohsen, Tian Furong, Kobayashi Hisatoshi, Tabata Yasuhiko
International Center for Young Scientists, National Institute for Materials Science, Ibaraki, Japan.
Tissue Eng. 2007 Jan;13(1):11-9. doi: 10.1089/ten.2006.0120.
The objective of this study was to create a novel approach to promote bone induction through sustained release of growth factor from a 3-dimensional (3D) hybrid scaffold. Peptide-amphiphile (PA) was synthesized by standard solid-phase chemistry that ends with the alkylation of the NH2 terminus of the peptide. Collagen sponge was reinforced by incorporation of poly(glycolic acid) (PGA) fiber. A 3D network of nanofibers was formed by mixing basic fibroblast growth factor (bFGF) suspensions with dilute aqueous solutions of PA. A hybrid scaffold was fabricated by combination of self-assembled PA nanofibers and collagen sponge reinforced with incorporation of PGA fibers. The in vitro release profile of bFGF from hybrid scaffold was investigated, and ectopic bone formation induced by the released bFGF was assessed after subcutaneous implantation of hybrid scaffold into the backs of rats. Homogeneous bone formation was histologically observed throughout the hybrid scaffolds, in marked contrast to collagen sponge-incorporated bFGF. The level of alkaline phosphatase activity and osteocalcin content at the implanted sites of hybrid scaffolds were significantly high compared with collagen sponge incorporated with bFGF. The combination of bFGF incorporated in a collagen sponge self-assembled PA nanofiber hybrid scaffold is a promising procedure to improve bone regeneration.
本研究的目的是创建一种新方法，通过从三维（3D）混合支架中持续释放生长因子来促进骨诱导。肽两亲分子（PA）通过标准固相化学合成，以肽的NH2末端烷基化结束。通过掺入聚乙醇酸（PGA）纤维增强胶原海绵。通过将碱性成纤维细胞生长因子（bFGF）悬浮液与PA稀水溶液混合形成纳米纤维三维网络。通过自组装PA纳米纤维与掺入PGA纤维增强的胶原海绵相结合制备混合支架。研究了bFGF从混合支架中的体外释放曲线，并在将混合支架皮下植入大鼠背部后评估释放的bFGF诱导的异位骨形成。组织学观察到整个混合支架中均有均匀的骨形成，这与掺入bFGF的胶原海绵形成明显对比。与掺入bFGF的胶原海绵相比，混合支架植入部位的碱性磷酸酶活性水平和骨钙素含量显著更高。将bFGF掺入胶原海绵自组装PA纳米纤维混合支架中的组合是改善骨再生的一种有前景的方法。
