Hosseinkhani Hossein, Hosseinkhani Mohsen, Kobayashi Hisatoshi
International Center for Young Scientists, National Institute for Materials Science, Nano and Biomaterials Research Building, Tsukuba, Ibaraki 305-0044, Japan.
Biomed Mater. 2006 Mar;1(1):8-15. doi: 10.1088/1748-6041/1/1/002. Epub 2006 Mar 1.
The objective of this study is to enhance the proliferation and differentiation of mesenchymal stem cells (MSC) in nanodimensional scaffolds. The proliferation and differentiation of MSC was investigated in a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile (PA) molecules. PA was synthesized by standard solid phase chemistry that ends with the alkylation of the NH(2) terminus of the peptide. The sequence of arginine-glycine-aspartic acid was included in the peptide design as well. A three-dimensional network of nanofibers was formed by mixing MSC suspensions in media with dilute aqueous solution of PA. The attachment, proliferation and osteogenic differentiation of MSC were influenced by the self-assembled PA nanofibers as the cell scaffold and the values were significantly high compared with those in the static culture. The alkaline phosphatase activity and osteocalcin content of MSC cultured in the PA nanofibers significantly increased compared with the static culture method. It may be concluded that PA nanofibers enable MSC to positively improve the proliferation and differentiation extent.
本研究的目的是增强间充质干细胞（MSC）在纳米尺寸支架中的增殖和分化。在由肽两亲分子（PA）自组装形成的纳米纤维三维网络中研究了MSC的增殖和分化。PA通过标准固相化学合成，以肽的NH(2)末端烷基化结束。肽设计中还包含精氨酸-甘氨酸-天冬氨酸序列。通过将MSC悬浮液与PA稀水溶液在培养基中混合形成纳米纤维三维网络。自组装的PA纳米纤维作为细胞支架影响了MSC的附着、增殖和成骨分化，与静态培养相比，这些值显著更高。与静态培养方法相比，在PA纳米纤维中培养的MSC的碱性磷酸酶活性和骨钙素含量显著增加。可以得出结论，PA纳米纤维能够使MSC积极提高增殖和分化程度。
