Stokols Shula, Sakamoto Jeff, Breckon Chris, Holt Todd, Weiss James, Tuszynski Mark H
Department of Bioengineering, University of California-San Diego, La Jolla, California 92093, USA.
Tissue Eng. 2006 Oct;12(10):2777-87. doi: 10.1089/ten.2006.12.2777.
While several strategies can stimulate axonal regeneration within a site of spinal cord injury, the growth of axons is generally disorganized and random. Biocompatible scaffolds that guide and maintain the native organization of axons regenerating through an injury site could be of importance in enhancing recovery of the nervous system after injury. Here we report a novel fabrication process for templated agarose nerve guidance scaffolds composed of uniaxial channels of precise diameter and wall thickness extending through their full length. When tested in an in vivo model of spinal cord injury, scaffolds exhibit excellent integration with host tissue and support linear axonal growth through their channels. Further, when loaded with bone marrow stromal cells genetically engineered to secrete brain-derived neurotrophic factor (BDNF), the number of linear penetrating axons is significantly enhanced. The templating process can be useful in fabricating nerve guidance scaffolds for both central and peripheral nerve injuries, or any materials application requiring a precise array of linearly oriented channels.
虽然有几种策略可以刺激脊髓损伤部位的轴突再生，但轴突的生长通常是杂乱无章且随机的。能够引导并维持通过损伤部位再生的轴突自然排列的生物相容性支架，对于促进损伤后神经系统的恢复可能具有重要意义。在此，我们报告一种新型的模板化琼脂糖神经引导支架制造工艺，该支架由精确直径和壁厚的单轴通道组成，通道贯穿其全长。当在脊髓损伤的体内模型中进行测试时，支架与宿主组织表现出良好的整合，并支持轴突通过其通道进行线性生长。此外，当加载经基因工程改造以分泌脑源性神经营养因子（BDNF）的骨髓基质细胞时，线性穿透轴突的数量会显著增加。这种模板化工艺可用于制造用于中枢和周围神经损伤的神经引导支架，或任何需要精确排列线性定向通道的材料应用。
