Helder Marco N, Knippenberg Marlene, Klein-Nulend Jenneke, Wuisman Paul I J M
Department of Orthopedic Surgery, VU University Medical Center (VUMC), Amsterdam, The Netherlands.
Tissue Eng. 2007 Aug;13(8):1799-808. doi: 10.1089/ten.2006.0165.
The perspective of an innovative new concept integrating tissue-engineering techniques with an established surgical technique is described. The focus is primarily on a one-step surgical procedure using adipose tissue-derived mesenchymal stem cells, a calcium phosphate scaffold as a carrier, and a bioresorbable polymer cage to facilitate spinal interbody fusion. We address the harvesting and processing of clinically relevant quantities of adipose tissue-derived mesenchymal stem cells, triggering of these stem cells toward lineage-specific differentiation, seeding of the triggered stem cells on a bioresorbable scaffold, and implantation of the resulting tissue-engineered construct. The integrated steps can be accomplished within one surgical procedure in a surgical theater. Although the proposed concept has been developed for spinal fusion, potential application in other surgical disciplines is presumed realistic.
本文描述了一种将组织工程技术与成熟外科技术相结合的创新概念。重点主要在于一种一步式外科手术，该手术使用脂肪组织来源的间充质干细胞、磷酸钙支架作为载体以及生物可吸收聚合物笼来促进脊柱椎间融合。我们阐述了临床相关数量的脂肪组织来源间充质干细胞的采集和处理、这些干细胞向特定谱系分化的触发、将触发后的干细胞接种到生物可吸收支架上以及植入所得的组织工程构建体。这些整合步骤可在手术室的一次外科手术中完成。尽管所提出的概念是为脊柱融合而开发的，但推测其在其他外科领域的潜在应用是现实可行的。
