Yanming S, Ruofeng Y, Muleke C I, Guangwei Z, Lixin X, Xiangrui L
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Parasite Immunol. 2007 Jun;29(6):319-26. doi: 10.1111/j.1365-3024.2007.00949.x.
The efficacy of vaccination against Haemonchus contortus infection with two recombinant proteins, rHco-gal-m and rHco-gal-f, was studied in 9-10-month-old goats. Vaccination with 100 microg protein reduced faecal egg output and worm burdens by 37.25% and 41.1%, respectively. Corresponding reductions with 200 microg protein were 48.03% and 46.19%. Vaccinated groups had significantly higher IgG levels than the negative and positive controls. Significant negative correlations were detected between IgG level, mucosal homogenate IgA concentration, haemoglobin and abomasal worm burden at necropsy. By contrast a positive correlation was found between the percentage of B cells, monocytes and abomasal worm burden. These findings suggested that vaccination with a combination of recombinant rHco-gal-m/f proteins had a role in protecting goats against H. contortus infection.
在9至10月龄的山羊中研究了用两种重组蛋白rHco-gal-m和rHco-gal-f接种疫苗对抗捻转血矛线虫感染的效果。接种100微克蛋白可使粪便虫卵排出量和虫负荷分别降低37.25%和41.1%。接种200微克蛋白时相应的降低率分别为48.03%和46.19%。接种组的IgG水平显著高于阴性和阳性对照组。尸检时，IgG水平、粘膜匀浆IgA浓度、血红蛋白与皱胃虫负荷之间存在显著负相关。相比之下，B细胞、单核细胞百分比与皱胃虫负荷之间呈正相关。这些发现表明，联合接种重组rHco-gal-m/f蛋白在保护山羊免受捻转血矛线虫感染方面发挥了作用。
