College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Vet J. 2012 Jan;191(1):94-100. doi: 10.1016/j.tvjl.2010.12.023. Epub 2011 Feb 16.
DNA vaccines expressing Haemonchus contortus H11 antigen with or without interleukin (IL)-2 were tested for protection against H. contortus infection in goats. Sixteen goats (8-10 months of age) were allocated into four trial groups. On days 0 and 14, group 1 was immunised with a DNA vaccine expressing H11 and IL-2 and group 2 was immunised with a DNA vaccine expressing H11 only. Group 3 was an unvaccinated positive control group challenged with H. contortus third stage larvae (L3). Group 4 was an unvaccinated negative control group that was not challenged with L3. Animals in groups 1-3 were challenged with 5000 infective H. contortus L3 14 days after the second immunisation. Transcription of H11 and IL-2 was demonstrated in muscle by reverse transcriptase-PCR 10 days after primary immunisation and translation of H11 was detected by Western blot analysis 7 days after the second immunisation. Following immunisation with a DNA vaccine expressing H11 and IL-2, high levels of specific serum immunoglobulin (Ig) G, non-specific serum IgA, mucosal IgA, CD4(+) T lymphocytes, CD8(+) T lymphocytes and B lymphocytes were produced. Following challenge with L3, cumulative mean faecal worm egg counts and worm burdens in group 1 were reduced by 56.6% and 46.7%, respectively, while corresponding reductions in group 2 were 44.8% and 38.0%. There was a small but significant difference in abomasal worm burdens in goats in groups 1 (395.3±37.6) and 2 (459.5±101.6) compared to group 3 (741.5±241.5; P<0.05). Use of a DNA vaccine expressing H11 and IL-2 conferred partial protection against Haemonchus contortus infection in goats.
用表达旋毛虫 H11 抗原的 DNA 疫苗联合或不联合白细胞介素（IL）-2 对山羊进行旋毛虫感染的保护作用试验。将 16 只山羊（8-10 月龄）分为 4 个试验组。第 1 天和第 14 天，第 1 组用表达 H11 和 IL-2 的 DNA 疫苗免疫，第 2 组用仅表达 H11 的 DNA 疫苗免疫。第 3 组为未接种的阳性攻毒对照组，用旋毛虫第 3 期幼虫（L3）攻毒。第 4 组为未接种的阴性对照组，未用 L3 攻毒。第 1-3 组动物在第 2 次免疫后 14 天用 5000 条感染性旋毛虫 L3 攻毒。初次免疫后 10 天，通过逆转录-PCR 显示肌肉中 H11 和 IL-2 的转录，第 2 次免疫后 7 天通过 Western blot 分析检测 H11 的翻译。用表达 H11 和 IL-2 的 DNA 疫苗免疫后，产生高水平的特异性血清免疫球蛋白（Ig）G、非特异性血清 IgA、黏膜 IgA、CD4+T 淋巴细胞、CD8+T 淋巴细胞和 B 淋巴细胞。用 L3 攻毒后，第 1 组累积平均粪便虫卵计数和虫体负荷分别减少 56.6%和 46.7%，第 2 组分别减少 44.8%和 38.0%。与第 3 组（741.5±241.5）相比，第 1 组（395.3±37.6）和第 2 组（459.5±101.6）的山羊网胃虫体负荷有较小但显著差异（P<0.05）。表达 H11 和 IL-2 的 DNA 疫苗可对山羊的旋毛虫感染提供部分保护。
