Futaki N, Arai I, Sugimoto M, Honma Y, Hashimoto Y, Sakurai T, Nakanishi Y, Tanaka M, Nakaike S
Pharmacology Laboratory, Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd, Kita-ku, Saitama-shi, Saitama, Japan.
Exp Dermatol. 2007 Jun;16(6):507-12. doi: 10.1111/j.1600-0625.2007.00548.x.
The role of prostaglandins (PGs) on mechanical scratching-induced cutaneous barrier disruption in mice was investigated by comparing the observed effects of arachidonic acid (AA) application. Scratching of the mouse skin with a stainless-steel wire brush (mechanical scratching) was associated with significant, scratch-count-dependent elevation of the transepidermal water loss (TEWL) and skin PG levels (especially PGD(2) and PGE(2)). Histological evidence of inflammation (crusta, acanthosis and neutrophilic infiltration) in the skin also became evident 24 h after mechanical scratching. On the other hand, while topical application of 0.1% AA to the mouse skin also increased the skin PG levels, but did not produce any increase of TEWL or histological evidence of inflammation in the skin. Topical application of cyclooxygenase inhibitors (indomethacin, piroxicam, aspirin, diclofenac and ketoprofen) decreased the spontaneous recovery rates from cutaneous barrier disruption. These results suggest that the elevation of cutaneous PG production induced by mechanical scratching is involved in the repair of the skin damage caused by the scratching.
通过比较花生四烯酸（AA）应用的观察效果，研究了前列腺素（PGs）在小鼠机械性搔抓诱导的皮肤屏障破坏中的作用。用不锈钢丝刷搔抓小鼠皮肤（机械性搔抓）与经表皮水分流失（TEWL）和皮肤PG水平（尤其是PGD₂和PGE₂）显著的、与搔抓次数相关的升高有关。皮肤炎症的组织学证据（结痂、棘皮症和嗜中性粒细胞浸润）在机械性搔抓后24小时也变得明显。另一方面，虽然将0.1% AA局部应用于小鼠皮肤也会增加皮肤PG水平，但不会使TEWL增加，也不会产生皮肤炎症的组织学证据。局部应用环氧化酶抑制剂（吲哚美辛、吡罗昔康、阿司匹林、双氯芬酸和酮洛芬）会降低皮肤屏障破坏后的自发恢复率。这些结果表明，机械性搔抓诱导的皮肤PG生成增加参与了搔抓所致皮肤损伤的修复。
