El-Shazly S, Mustafa A S, Ahmad S, Al-Attiyah R
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Int J Tuberc Lung Dis. 2007 Jun;11(6):676-82.
The mammalian cell entry (mce) proteins Mce3A, Mce3D and Mce3E, encoded by the mce3 operon of Mycobacterium tuberculosis, have recently been shown to be expressed during natural infection in humans.
To determine the potential of Mce3A, Mce3D and Mce3E proteins in the serodiagnosis of tuberculosis (TB).
The quantitative detection of anti-Mce3A, -Mce3D and -Mce3E antibodies in serum samples from active TB patients (n = 58), healthy contacts of TB patients (n = 24) and bacilli Calmette-Guérin (BCG) vaccinated healthy subjects (n = 24) was performed using enzyme-linked immunosorbent assay (ELISA).
Antibodies in serum from 98%, 86% and 90% of active TB patients and from 92%, 75% and 96% of healthy contacts of TB patients reacted with Mce3A, Mce3D and Mce3E proteins, respectively. However, none of the serum from BCG-vaccinated healthy subjects reacted with Mce3A and Mce3E proteins, and only 8% of serum samples reacted with Mce3D protein. Overall, serum from 98% active TB patients, 96% healthy contacts and 0% BCG-vaccinated healthy subjects were positive for anti-Mce3A and/or -Mce3E antibodies.
Our results suggest that Mce3A and Mce3E proteins may be useful for the serodiagnosis of TB infection.
由结核分枝杆菌的mce3操纵子编码的哺乳动物细胞进入（mce）蛋白Mce3A、Mce3D和Mce3E最近已被证明在人类自然感染期间表达。
确定Mce3A、Mce3D和Mce3E蛋白在结核病血清学诊断中的潜力。
采用酶联免疫吸附试验（ELISA）对活动性肺结核患者（n = 58）、肺结核患者的健康接触者（n = 24）和接种卡介苗（BCG）的健康受试者（n = 24）血清样本中的抗Mce3A、抗Mce3D和抗Mce3E抗体进行定量检测。
分别有98%、86%和90%的活动性肺结核患者血清以及92%、75%和96%的肺结核患者健康接触者血清中的抗体与Mce3A、Mce3D和Mce3E蛋白发生反应。然而，接种卡介苗的健康受试者血清中无一与Mce3A和Mce3E蛋白发生反应，仅有8%的血清样本与Mce3D蛋白发生反应。总体而言，98%的活动性肺结核患者血清、96%的肺结核患者健康接触者血清以及0%的接种卡介苗健康受试者血清中抗Mce3A和/或抗Mce3E抗体呈阳性。
我们的结果表明，Mce3A和Mce3E蛋白可能有助于结核病感染的血清学诊断。
