Gao Shuping, De Geyter Christian, Kossowska K, Zhang Hong
Department of Research, Women's Hospital, University of Basel, Switzerland.
Mol Hum Reprod. 2007 Jul;13(7):465-71. doi: 10.1093/molehr/gam037. Epub 2007 May 22.
We report the characterization of full-length human ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-16, a novel member of the disintegrin and metalloproteinase with thrombospondin motifs (hence ADAMTS) family. ADAMTS-16 is highly expressed both in the kidney and in the ovary, where it is predominantly expressed in the parietal granulosa cells of pre-ovulatory follicles but only slightly expressed in cells of the cumulus oophorus. In fully differentiated luteinizing granulosa cells, follicle-stimulating hormone and forskolin induces expression of ADAMTS-16, suggesting that it is regulated via the cAMP pathway. Luteinizing hormone had a minor effect on the expression of ADAMTS-16. ADAMTS-16 is capable of cleaving alpha2-macroglobulin (MG), a common substrate for proteases, which is present at high concentrations in the follicular fluid of ovarian follicles. These studies provide the first evidence that ADAMTS-16 is an active protease and suggest a physiological role of ADAMTS-16 in ovarian follicles, at least during the pre-ovulatory phase.
我们报告了全长人ADAMTS（含血小板反应蛋白基序的解聚素和金属蛋白酶）-16的特性，它是含血小板反应蛋白基序的解聚素和金属蛋白酶（即ADAMTS）家族的一个新成员。ADAMTS-16在肾脏和卵巢中均高表达，在卵巢中它主要在排卵前卵泡的颗粒细胞中表达，而在卵丘细胞中仅少量表达。在完全分化的黄体化颗粒细胞中，促卵泡激素和福斯高林可诱导ADAMTS-16的表达，这表明它是通过cAMP途径调控的。黄体生成素对ADAMTS-16的表达影响较小。ADAMTS-16能够切割α2-巨球蛋白（MG），MG是蛋白酶的一种常见底物，在卵巢卵泡的卵泡液中高浓度存在。这些研究首次证明ADAMTS-16是一种活性蛋白酶，并提示ADAMTS-16在卵巢卵泡中至少在排卵前阶段具有生理作用。
