Furuya Masataka, Tanaka Mamoru, Teranishi Takahide, Matsumoto Kazuya, Hosoi Yoshihiko, Saeki Kazuhiro, Ishimoto Hitoshi, Minegishi Kazuhiro, Iritani Akira, Yoshimura Yasunori
Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
J Reprod Dev. 2007 Aug;53(4):895-902. doi: 10.1262/jrd.19008. Epub 2007 May 23.
Oocyte-specific linker histone H1foo is localized in the oocyte nucleus, either diffusely or bound to chromatin, during the processes of meiotic maturation and fertilization. This expression pattern suggests that H1foo plays a key role in the control of gene expression and chromatin modification during oogenesis and early embryogenesis. To reveal the function of H1foo, we microinjected antisense morpholino oligonucleotides (MO) against H1foo into mouse germinal-vesicle stage oocytes. The rate of in vitro maturation of the antisense MO group was significantly lower than that of the control group. Eggs that failed to extrude a first polar body following injection of antisense MO arrested at metaphase I. Additionally, co-injection of in vitro synthesized H1foo mRNA along with antisense MO successfully rescued expression of H1foo and improved the in vitro maturation rate. There was no difference in the rate of parthenogenesis between the antisense MO and control groups. These results indicate that H1foo is essential for maturation of germinal vesicle-stage oocytes.
在减数分裂成熟和受精过程中，卵母细胞特异性连接组蛋白H1foo定位于卵母细胞核内，呈弥散状或与染色质结合。这种表达模式表明，H1foo在卵子发生和早期胚胎发育过程中基因表达调控和染色质修饰方面发挥关键作用。为揭示H1foo的功能，我们将针对H1foo的反义吗啉代寡核苷酸（MO）显微注射到小鼠生发泡期卵母细胞中。反义MO组的体外成熟率显著低于对照组。注射反义MO后未能排出第一极体的卵母细胞停滞在中期I。此外，将体外合成的H1foo mRNA与反义MO共同注射可成功挽救H1foo的表达并提高体外成熟率。反义MO组和对照组的孤雌生殖率没有差异。这些结果表明，H1foo对于生发泡期卵母细胞的成熟至关重要。
