Ayoub S, Berson G, Rock E, Younes A
INSERM U. 195, Faculté de Medecine, Université de Clermont-Ferrand, France.
Cell Biochem Funct. 1991 Jul;9(3):193-9. doi: 10.1002/cbf.290090307.
ATP translocation into mitochondria isolated from halothane-sensitive pig (HP) muscle was dramatically reduced compared with normal pigs (NP). To determine if this was due to a decreased amount of ATP translocase in the mitochondrial membranes, or a structural modification of this protein, an electrophoretic study was undertaken. Total proteins and purified translocase preparations from (NP) and (HP) mitochondria were analyzed by SDS gel electrophoresis. In the two types of mitochondria no significant differences were observed either in the amount of ATP translocase or in the molecular weight. Also, neither nonequilibrium pH gradient gel electrophoresis nor the analysis of peptides produced by limited proteolysis revealed any structural difference between the two types of protein. On the basis of these results, the depressed translocase activity observed in (HP) mitochondria cannot be explained by a reduced amount of the nucleotide translocase, nor a structural alteration of this protein. Possible inhibition of (HP) translocase activity by Ca2+ accumulation or by other mechanisms is discussed.
与正常猪（NP）相比，从氟烷敏感猪（HP）肌肉中分离出的线粒体对ATP的转运显著减少。为了确定这是由于线粒体膜中ATP转位酶数量减少，还是该蛋白质的结构改变所致，进行了一项电泳研究。通过SDS凝胶电泳分析了来自（NP）和（HP）线粒体的总蛋白和纯化的转位酶制剂。在这两种类型的线粒体中，无论是ATP转位酶的数量还是分子量都没有观察到显著差异。此外，非平衡pH梯度凝胶电泳和有限蛋白酶解产生的肽段分析均未揭示这两种类型蛋白质之间的任何结构差异。基于这些结果，在（HP）线粒体中观察到的转位酶活性降低不能用核苷酸转位酶数量减少或该蛋白质的结构改变来解释。本文讨论了Ca2+积累或其他机制对（HP）转位酶活性的可能抑制作用。
