Chen Shuai, Zhang Mingjun, Ma Honghui, Saiyin Hexige, Shen Suqin, Xi Jiajie, Wan Bo, Yu Long
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
Cancer Chemother Pharmacol. 2008 Mar;61(3):459-69. doi: 10.1007/s00280-007-0491-y. Epub 2007 May 23.
Cyclophilin A (CYPA) belongs to peptidyl prolyl isomerases (PPIases), which catalyze the cis/trans isomerization of prolyl peptide bonds in cellular communication. CYPA has been implicated in several pathological processes, including cancer, inflammatory diseases, and HIV-1 infection. Up-regulation of CYPA has been found to be a common phenomenon in several tumor types, including in hepatocellular carcinoma (HCC). However, the role of CYPA in tumor cells remains unknown. We generated a stable SK-Hep1 cell line and studied the CYPA regulated genes at the transcriptome level. The microarray results reveal that CYPA can up-regulate the expression of many cytokine and drug resistance related genes. Furthermore, we showed that the elevated CYPA expression contributes to drug resistance. We postulate that the over-expression of CYPA in tumors may play a role in clinical resistance to chemotherapy.
亲环素A（CYPA）属于肽基脯氨酰异构酶（PPIase），其在细胞通讯中催化脯氨酰肽键的顺/反异构化。CYPA参与了多种病理过程，包括癌症、炎症性疾病和HIV-1感染。在包括肝细胞癌（HCC）在内的多种肿瘤类型中，CYPA的上调是一种常见现象。然而，CYPA在肿瘤细胞中的作用仍不清楚。我们构建了一个稳定的SK-Hep1细胞系，并在转录组水平研究了CYPA调控的基因。微阵列结果显示，CYPA可上调许多细胞因子和耐药相关基因的表达。此外，我们表明CYPA表达升高会导致耐药。我们推测肿瘤中CYPA的过表达可能在临床化疗耐药中起作用。
