[荧光多重聚合酶链反应检测结直肠癌微卫星不稳定性及其临床价值]

[Detection of microsatellite instability in colorectal cancer by fluorescence multiplex polymerase chain reaction and its clinical value].

作者信息

Jin Hei-ying, Lai Ren-sheng, Ding Yi-jiang, Xie Ling, Yang Bo-lin, Liu Fei, Ding Shu-qing, Ge Yong-sheng

机构信息

National Center of Colorectal Surgery, The Third Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210001, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2007 May;10(3):217-20.

PMID:17520377

Abstract

OBJECTIVE

To detect microsatellite instability(MSI) in colorectal cancer by fluorescence multiplex polymerase chain reaction(FM-PCR) and explore its clinical value.

METHODS

MSI of 110 colorectal cancer patients undergone surgical resection in our department from 2004 to 2005 were examined by FM-PCR, and the pathological characteristics were compared between MSI and microsatellite stable (MSS) colorectal cancer patients.

RESULTS

Among 110 cases, the male were 66 and the female were 44. Mean age was 60.8 (26-94) yrs. All 5 microsatellite markers were amplified. Out of them, 10 cases (8.1%) were MSI-H, 13 cases (11.8%) were MSI-L and 87 cases (79.1%) were MSS. Instability of BAT-26 was found in 9 cases (8.2%), BAT-25 was in 11 cases (10.0%), D2S123 was in 11 cases (10.0%), D5S346 was in 6 cases (8.2%) and D17S250 was in 8 cases (7.3%). Age between MSI and MSS colorectal cancer patients was significant and other pathological characteristics were not significant.

CONCLUSIONS

FM-PCR is a clinically stable method for MSI detection in colorectal cancer patients. There are no significant differences between MSI and MSS pathological characteristics of colorectal cancer patients.

摘要

目的

采用荧光多重聚合酶链反应（FM-PCR）检测结直肠癌中的微卫星不稳定性（MSI），并探讨其临床价值。

方法

对2004年至2005年在我科接受手术切除的110例结直肠癌患者进行FM-PCR检测MSI，并比较MSI和微卫星稳定（MSS）结直肠癌患者的病理特征。

结果

110例患者中，男性66例，女性44例。平均年龄60.8（26 - 94）岁。所有5个微卫星标记均被扩增。其中，10例（8.1%）为高度微卫星不稳定（MSI-H），13例（11.8%）为低度微卫星不稳定（MSI-L），87例（79.1%）为微卫星稳定（MSS）。BAT-26不稳定9例（8.2%），BAT-25不稳定11例（10.0%），D2S123不稳定11例（10.0%），D5S346不稳定6例（8.2%），D17S250不稳定8例（7.3%）。MSI和MSS结直肠癌患者的年龄差异有统计学意义，其他病理特征差异无统计学意义。