Ch'ng Eweseng, Tomita Yasuhiko, Zhang Binglin, He Juxiang, Hoshida Yoshihiko, Qiu Ying, Morii Eiichi, Nakamichi Itsuko, Hamada Ken-ichiro, Ueda Takafumi, Aozasa Katsuyuki
Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Cancer. 2007 Jul 1;110(1):164-72. doi: 10.1002/cncr.22764.
CD100, a class IV semaphorin, promotes angiogenesis, invasive growth, proliferation, and antiapoptosis of cancer cells in vitro. The expression of CD100 in soft tissue sarcoma (STS) and its correlation with clinicopathologic factors and prognostic significance were evaluated.
Expression levels of CD100 in patients with localized STS were evaluated immunohistochemically on paraffin-embedded sections from 81 patients, including 47 men and 34 women with a median age of 54 years. Staining intensity was categorized into weaker than (level 1) or equal to that of lymphocytes with a rate of <10% stained tumor cells (level 2) or >10% stained tumor cells (level 3). Ki-67 staining was performed in parallel.
Forty-two tumors (52%) had level 1 CD100 expression, 18 tumors (22%) had level 2 CD100 expression, and 21 tumors (26%) had level 3 CD100 expression. Tumors that had level 2 and 3 CD100 expression were correlated significantly with higher mitotic count, cellularity, ratio of necrosis, and Ki-67 labeling index (LI) compared with tumors that had level 1 CD100 expression. There was no correlation between CD100 expression and other characteristics. Among the 3 levels of CD100 expression, higher expression levels were correlated with poorer overall and disease-free survival. Multivariate analysis revealed that CD100 expression (levels 1 and 2 vs level 3) and tumor size (<or=5 cm vs >5 cm) were independent prognosticators for overall survival (P < .05 for both), and CD100 expression (levels 1 and 2 vs level 3) was an independent prognosticator for disease-free survival (P < .05).
The results from this study indicated the demonstrated prognostic significance of CD100 expression in STS.
CD100是一种IV类信号素，在体外可促进癌细胞的血管生成、侵袭性生长、增殖及抗凋亡。本研究评估了CD100在软组织肉瘤（STS）中的表达情况及其与临床病理因素的相关性和预后意义。
采用免疫组织化学方法对81例局限性STS患者石蜡包埋切片中CD100的表达水平进行评估，其中男性47例，女性34例，中位年龄54岁。染色强度分为弱于淋巴细胞（1级）、等于淋巴细胞且染色肿瘤细胞率<10%（2级）或>10%（3级）。同时进行Ki-67染色。
42例肿瘤（52%）为1级CD100表达，18例肿瘤（22%）为2级CD100表达，21例肿瘤（26%）为3级CD100表达。与1级CD100表达的肿瘤相比，2级和3级CD100表达的肿瘤与较高的有丝分裂计数、细胞密度、坏死率及Ki-67标记指数（LI）显著相关。CD100表达与其他特征之间无相关性。在CD100表达的3个水平中，较高的表达水平与较差的总生存期和无病生存期相关。多因素分析显示，CD100表达（1级和2级 vs 3级）和肿瘤大小（≤5 cm vs >5 cm）是总生存期的独立预后因素（两者P均<.05），CD100表达（1级和2级 vs 3级）是无病生存期的独立预后因素（P<.05）。
本研究结果表明CD100表达在STS中具有预后意义。
