Jibu T, Ando K, Matsumoto T, Koike S, Kobori O, Morioka Y, Kanegasaki S
First Department of Surgery, School of Medicine, University of Tokyo, Japan.
Clin Exp Metastasis. 1991 Nov-Dec;9(6):529-40. doi: 10.1007/BF01768581.
We have previously reported that abdominal irradiation of mice inhibited lung metastases of a weakly immunogenic fibrosarcoma, and that transmigration after the irradiation of Enterobacter cloacae into mesenteric lymph nodes coincided with this phenomenon. In this paper, we show that Escherichia coli as well as E. cloacae reduce the number of metastatic lung colonies when these bacteria were intravenously injected into mice prior to the tumour cell challenge. The inhibition was caused not only by the administration of living bacteria but also by that of killed bacteria. Bacterial lipopolysaccharide (LPS), a component of membrane, replaced at least in part the effect of whole bacteria. Transfer of spleen cells from LPS-treated mice into intact recipients prominently inhibited metastatic development in the recipient mice. 'Cross transfer' between LPS high responders and LPS low responders suggested an indirect activity of transferred spleen cells. The antimetastatic activity of LPS depended on the tumour cell type; metastasis of fibrosarcomas was extensively inhibited by LPS irrespective of tumour immunogenicity while that of adenocarcinomas was only slightly inhibited. These results suggest that non-immunological mechanisms are involved in the antimetastatic activity of LPS.
我们之前曾报道，对小鼠进行腹部照射可抑制低免疫原性纤维肉瘤的肺转移，并且阴沟肠杆菌照射后向肠系膜淋巴结的迁移与这一现象相符。在本文中，我们表明，在肿瘤细胞攻击之前将大肠杆菌以及阴沟肠杆菌静脉注射到小鼠体内时，它们会减少肺转移瘤集落的数量。这种抑制不仅由活细菌的给药引起，也由死细菌的给药引起。细菌脂多糖（LPS），一种细胞膜成分，至少部分替代了全细菌的作用。将来自LPS处理小鼠的脾细胞转移到未处理的受体小鼠中，显著抑制了受体小鼠的转移发展。LPS高反应者和LPS低反应者之间的“交叉转移”表明转移的脾细胞具有间接活性。LPS的抗转移活性取决于肿瘤细胞类型；无论肿瘤免疫原性如何，LPS都能广泛抑制纤维肉瘤的转移，而对腺癌的转移仅略有抑制。这些结果表明，非免疫机制参与了LPS的抗转移活性。
