Claesson K, Lindell S, Southard J H, Belzer F O
University of Wisconsin Hospital, Department of Surgery, Madison 53792.
Cryobiology. 1991 Oct;28(5):422-7. doi: 10.1016/0011-2240(91)90050-x.
Rats were pretreated with a single iv dose of chlorpromazine (CPZ) 3 mg/kg, verapamil 1 mg/kg, or quinacrine 2 mg/kg. Livers were taken out and perfused with University of Wisconsin (UW) preservation solution and stored on ice for 48 h in the UW solution before reperfusion with erythrocyte-free and colloid-free Krebs-Hanseleit buffer at 38 degrees C in a nonrecirculating perfusion system for 2 h. CPZ- and quinacrine-pretreated livers produced significantly more bile than control livers and also released significantly less alanine aminotransferase into the perfusate at 30, 60, and 120 min of reperfusion. Aspartate aminotransferase levels were lower at 30 and 60 min of reperfusion for CPZ-pretreated livers but not at 120 min. The only difference between groups concerning lactate dehydrogenase (LDH) release into the perfusate was that CPZ decreased the amount of LDH released at 60 min. Total tissue water or tissue electrolyte content of the liver tissue at the end of the reperfusion did not differ between groups. In conclusion, verapamil was ineffective when given as single dose iv pretreatment to the liver donor but pretreatment with CPZ or quinacrine appeared to improve the function of the preserved liver.
大鼠预先静脉注射单次剂量的氯丙嗪（CPZ）3毫克/千克、维拉帕米1毫克/千克或奎纳克林2毫克/千克。取出肝脏，用威斯康星大学（UW）保存液灌注，然后在UW溶液中于冰上保存48小时，之后在38摄氏度的非循环灌注系统中用无红细胞和无胶体的克氏-亨氏缓冲液再灌注2小时。CPZ和奎纳克林预处理的肝脏产生的胆汁明显多于对照肝脏，并且在再灌注30、60和120分钟时向灌注液中释放的丙氨酸转氨酶也明显较少。CPZ预处理的肝脏在再灌注30和60分钟时天冬氨酸转氨酶水平较低，但在120分钟时并非如此。各组之间关于乳酸脱氢酶（LDH）释放到灌注液中的唯一差异是CPZ在60分钟时减少了LDH的释放量。再灌注结束时肝脏组织的总组织水分或组织电解质含量在各组之间没有差异。总之，维拉帕米作为肝脏供体的单次静脉预处理无效，但CPZ或奎纳克林预处理似乎可改善保存肝脏的功能。
