Jamieson N V, Sundberg R, Lindell S, Southard J H, Belzer F O
Department of Surgery, University of Wisconsin, Madison 53792.
Cryobiology. 1988 Aug;25(4):300-10. doi: 10.1016/0011-2240(88)90038-7.
Rabbit livers were stored cold for periods of 6 or 24 hr and tested using the isolated perfused liver model. Five solutions were tested: Eurocollins (EC), Ross and Marshall's hypertonic citrate (HC), modified plasma protein fraction (Cambridge PPF), Ringer lactate, and the recently developed "University of Wisconsin" (UW) solution. After storage livers were perfused with an erythrocyte-free oxygenated Krebs-Henseleit solution containing 4% bovine serum albumin at 38 degrees C for 2 hr. Bile production proved to be the most sensitive index of liver function for discriminating between the various storage solutions and the different preservation times. After 6 hr of cold storage, bile production was similar to control liver bile production (9.8 +/- 2.4 ml/2 hr/100 g) in livers stored in HC (8.8 +/- 2 ml), PPF (9.9 +/- 2.2 ml), and UW (10.3 +/- 1.9 ml); it was slightly depressed in EC (6.7 +/- 2.5 ml, P = 0.06), and markedly depressed in Ringer lactate (4.3 +/- 0.8 ml, P less than 0.05). After 24 hr of cold storage bile production in UW-stored livers was near normal (9.3 +/- 0.7 ml) but significantly depressed (3.5-6.2 ml) in all other solutions tested. Release of enzymes into the normothermic perfusate was also measured (aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase). In this small series the differences between cold storage solutions did not always reach statistical significance although the trend was for less enzyme release in livers stored in UW solution. This technique permits rapid assessment and refinement of new storage methods and new solutions for liver preservation prior to testing in a large animal transplant model. The results suggest that UW solution is superior to other preservation solutions and would permit successful 24-hr storage of livers.
将兔肝冷藏6小时或24小时，然后使用离体灌注肝模型进行检测。测试了五种溶液：欧氏液（EC）、罗斯和马歇尔的高渗柠檬酸盐溶液（HC）、改良血浆蛋白成分（剑桥PPF）、乳酸林格液和最近研制的“威斯康星大学”（UW）溶液。储存后，肝脏在38℃下用含4%牛血清白蛋白的无红细胞氧合克雷布斯-亨塞尔特溶液灌注2小时。胆汁生成被证明是区分各种储存溶液和不同保存时间的肝功能最敏感指标。冷藏6小时后，储存在HC（8.8±2毫升）、PPF（9.9±2.2毫升）和UW（10.3±1.9毫升）中的肝脏胆汁生成与对照肝脏胆汁生成（9.8±2.4毫升/2小时/100克）相似；在EC中略有下降（6.7±2.5毫升，P = 0.06），在乳酸林格液中明显下降（4.3±0.8毫升，P＜0.05）。冷藏24小时后，UW储存的肝脏胆汁生成接近正常（9.3±0.7毫升），但在所有其他测试溶液中显著下降（3.5 - 6.2毫升）。还测量了酶释放到常温灌注液中的情况（天冬氨酸转氨酶、丙氨酸转氨酶和乳酸脱氢酶）。在这个小样本系列中，尽管趋势是储存在UW溶液中的肝脏酶释放较少，但冷藏溶液之间的差异并不总是达到统计学显著性。该技术允许在大型动物移植模型中进行测试之前，快速评估和改进新的储存方法和肝脏保存新溶液。结果表明，UW溶液优于其他保存溶液，能够成功保存肝脏24小时。
