组织培养细胞中的胆固醇酯代谢：II. Fu5AH大鼠肝癌细胞中积累的酯化胆固醇的来源。

Cholesteryl ester metabolism in tissue culture cells: II. Source of accumulated esterified cholesterol in Fu5AH rat hepatoma cells.

作者信息

Rothblat G H, Arbogast L, Kritchevsky D, Naftulin M

出版信息

Lipids. 1976 Feb;11(2):97-108. doi: 10.1007/BF02532658.

Abstract

Previous investigations had demonstrated that Fu5AH rat hepatoma cells accumulated large quantities of esterified cholesterol when grown in hyperlipemic rabbit serum. The present investigation has determined the sources of the cellular esterified cholesterol when the cells were grown in hyperlipemic serum. Cellular esterification of endogenous and exogenous free cholesterol contributed 10% and 30%, respectively. The remaining 60% of the accumulated cellular esterfied cholesterol was derived from exogenous (serum) cholesteryl esters. Evidence for the hydrolysis of a portion of the incorporated esterified cholesterol is presented. A stimulation of free cholesterol incorporation and cellular esterification is elicited by hyperlipemic serum and serum lipoproteins when compared to normolipemic serum present at equivalent exogenous cholesterol concentrations. The effect of hyperlipemic serum is reduced by Tween-80 and Triton WR-1339. Comparative data on esterified cholesterol accumulation, free cholesterol incorporation, and cellular cholesterol esterification in Fu5-5 rat hepatoma cells, L-cells, and rabbit aortic medial cells are presented.

摘要

先前的研究表明，Fu5AH大鼠肝癌细胞在高脂血症兔血清中生长时会积累大量酯化胆固醇。本研究确定了细胞在高脂血症血清中生长时细胞酯化胆固醇的来源。内源性和外源性游离胆固醇的细胞酯化分别占10%和30%。积累的细胞酯化胆固醇的其余60%来自外源性（血清）胆固醇酯。本文提供了一部分掺入的酯化胆固醇发生水解的证据。与等外源性胆固醇浓度下的正常血脂血清相比，高脂血症血清和血清脂蛋白会引发游离胆固醇掺入和细胞酯化的增加。吐温80和曲拉通WR-1339可降低高脂血症血清的作用。本文还提供了Fu5-5大鼠肝癌细胞、L细胞和兔主动脉中膜细胞中酯化胆固醇积累、游离胆固醇掺入和细胞胆固醇酯化的比较数据。