[Toll样受体（TLR）配体对健康人和常见可变免疫缺陷患者外周血单个核细胞促炎细胞因子体外合成的影响]

[Effect of toll-like receptors (TLR) ligands on in vitro synthesis of proinflammatory cytokines by peripheral blood mononuclear cells from healthy persons and from patients with common variable immunodeficiency].

作者信息

Koval'chuk L V, Khoreva M V, Varivoda A S, Pashchenko O E, Gracheva L A, Bykova L P, Kondratenko I V, Bologov A A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2007 Jan-Feb(1):38-42.

Abstract

Production of citokines of tumor-necrosis factor (TNF)-alpha and interferon (IFN)-alpha by peripheral blood mononuclear cells (PBMC) from healthy men and from patients with common variable immunodeficiency (CVID) after stimulation with zymosan, lypopolysaccharides, flagellin and CpG, which are ligands of TLR 2/6, TLR 4, TLR 5, TLR 9 respectively, was studied in vitro. In healthy men production of TNF-alpha varied between individuals, whereas synthesis of IFN-alpha was similar. Spontaneous production of TNF-alpha by PBMC in patients with CVID was increased and accompanied by decrease in TNF-alpha production stimulated by each analyzed ligands except CpG. Observed changes in TLR-dependent TNF-alpha production can play important role in pathogenesis of CVID.

摘要

体外研究了来自健康男性和常见可变免疫缺陷（CVID）患者的外周血单个核细胞（PBMC）在用酵母聚糖、脂多糖、鞭毛蛋白和CpG刺激后肿瘤坏死因子（TNF）-α和干扰素（IFN）-α细胞因子的产生情况，这些分别是Toll样受体（TLR）2/6、TLR 4、TLR 5、TLR 9的配体。在健康男性中，TNF-α的产生在个体间存在差异，而IFN-α的合成则相似。CVID患者PBMC自发产生的TNF-α增加，并且除CpG外，每种分析配体刺激后TNF-α的产生均减少。观察到的TLR依赖性TNF-α产生的变化可能在CVID的发病机制中起重要作用。