基于单克隆抗体的用于检测布鲁氏菌抗原的高灵敏度酶联检测法

[Highly sensitive enzyme-linked assay based on monoclonal antibodies for detection of Brucella antigens].

作者信息

Fel'dsherova A A, El'gort D A, Konopleva M V, Khats Iu S, Tret'iakov O Iu, Kulakov Iu K, Tolmacheva T A, Zheludkov M M, Suslov A P

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2007 Jan-Feb(1):52-7.

PMID:17523430

Abstract

Mice monoclonal antibodies against lypopolysaccharides (LPS) of Brucella abortus has been obtained and characterized. The antibodies detected LPS of B. abortus, B. melitensis and B. suis with high sensivity and specificity and did not react with LPS of Yersinia enterocolitica O:3, Y. enterocolitica O:9, Salmonella typhimurium, and Francisella tularensis. It has been shown that interaction of monoclonal antibodies and LPS of Brucella species can be critically dependent from buffer system. Obtained monoclonal antibodies allowed to develop highly sensitive assay which was able to detect antigens of Brucella species in concentrations 0.05 - 0.1 ng/ml. The assay can be used for detection and identification of Brucella species.

摘要

已获得并鉴定了针对流产布鲁氏菌脂多糖（LPS）的小鼠单克隆抗体。这些抗体能以高灵敏度和特异性检测流产布鲁氏菌、羊种布鲁氏菌和猪种布鲁氏菌的LPS，且不与小肠结肠炎耶尔森菌O:3、小肠结肠炎耶尔森菌O:9、鼠伤寒沙门氏菌和土拉弗朗西斯菌的LPS发生反应。研究表明，单克隆抗体与布鲁氏菌属LPS的相互作用可能严重依赖于缓冲系统。所获得的单克隆抗体可用于开发高灵敏度检测方法，该方法能够检测浓度为0.05 - 0.1 ng/ml的布鲁氏菌属抗原。该检测方法可用于布鲁氏菌属的检测和鉴定。