Vizcaíno N, Fernández-Lago L
Departamento de Microbiología y Genética, Facultad de Farmacia, Universidad de Salamanca, España.
Res Microbiol. 1992 Jun;143(5):513-8. doi: 10.1016/0923-2508(92)90098-9.
A coagglutination test using monoclonal antibody BmE10-5 with specificity for the M antigen of Brucella melitensis 16M has been developed for the rapid identification of the smooth Brucella species. All reference strains of several biovars of B. melitensis, B. abortus, B. suis and B. neotomae tested were positive in this assay. No significant differences in reaction intensity were observed in relation to the different distribution of the A and M antigens among the Brucella serovars analysed. Conversely, rough Brucella species, with the exception of B. abortus 45/20, were negative in the assay. Among the different organisms tested not belonging to the genus Brucella, serovar O9 of Yersinia enterocolitica was the only one that gave a weak positive reaction of coagglutination. Thus, in view of its rapidity, simplicity, specificity and low costs, this technique could be highly useful for rapid identification of smooth Brucella strains in diagnostic laboratories.
已开发出一种使用对布鲁氏菌16M M抗原具有特异性的单克隆抗体BmE10-5的协同凝集试验，用于快速鉴定光滑型布鲁氏菌种。对布鲁氏菌属几个生物变种的所有参考菌株，包括流产布鲁氏菌、猪布鲁氏菌和内华达布鲁氏菌进行检测，结果在该试验中均呈阳性。在所分析的布鲁氏菌血清型中，A抗原和M抗原的不同分布与反应强度之间未观察到显著差异。相反，粗糙型布鲁氏菌种，除了流产布鲁氏菌45/20外，在该试验中均为阴性。在测试的不属于布鲁氏菌属的不同生物体中，小肠结肠炎耶尔森氏菌血清型O9是唯一产生微弱协同凝集阳性反应的菌株。因此，鉴于其快速、简便、特异且成本低，该技术在诊断实验室中对于快速鉴定光滑型布鲁氏菌菌株可能非常有用。
