Solanki Pratima R, Arya Sunil K, Nishimura Y, Iwamoto M, Malhotra B D
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi-110012, India.
Langmuir. 2007 Jun 19;23(13):7398-403. doi: 10.1021/la700350x. Epub 2007 May 25.
Cholesterol oxidase has been covalently immobilized onto 11-amino-1-undecanethiol hydrochloride (AUT) self-assembled monolayer (SAM) fabricated on gold (Au) substrates using glutaraldehyde as a cross-linker. These ChOx/AUT/Au bioelectrodes characterized using contact angle (CA) measurements; electrochemical technique and atomic force microscopy (AFM) have been utilized for the estimation of cholesterol in solution using the surface plasmon resonance (SPR) technique. These biosensing electrodes exhibiting linearity from 50 to 500 mg/dL of cholesterol in solution and sensitivity of 1.23 m0/(mg dL), can be used more than 20 times and have a shelf life of about 10 weeks when stored at 4 degrees C.
胆固醇氧化酶已通过戊二醛作为交联剂共价固定在在金（Au）基底上制备的11-氨基-1-十一烷硫醇盐酸盐（AUT）自组装单分子层（SAM）上。这些ChOx/AUT/Au生物电极通过接触角（CA）测量进行表征；已利用电化学技术和原子力显微镜（AFM），采用表面等离子体共振（SPR）技术来测定溶液中的胆固醇。这些生物传感电极在溶液中胆固醇浓度为50至500mg/dL时呈现线性，灵敏度为1.23 m0/(mg dL)，可使用20多次，在4℃储存时保质期约为10周。
