Sparrow Rosemary L, Veale Margaret F, Healey Geraldine, Payne Katherine A
Research Unit, Australian Red Cross Blood Service, Melbourne, Victoria, Australia.
Transfusion. 2007 Jun;47(6):966-8. doi: 10.1111/j.1537-2995.2007.01230.x.
Membrane-associated carbohydrate changes act as signals for removal of senescent and damaged red blood cells (RBCs) from the circulation and could play a role in the RBC storage lesion and RBC survival after transfusion. In this study, a panel of lectins was used to investigate the expression of carbohydrates on RBCs that had been separated before storage into young and old RBCs.
Leukodepleted RBCs were separated before storage into young and old RBCs (n = 9 paired units) by centrifugation and sampled at nominated time points during 42 days of storage. Changes to carbohydrate expression at the RBC membrane during storage were determined by flow cytometry with a panel of fluorescein-labeled lectins.
Old RBCs showed lower fluorescence intensity throughout storage, suggesting reduced binding of lectins compared to young RBCs. Progressively increased binding of lectins specific for galactose and N-acetylglucosamine residues was observed during storage of young and old RBCs.
Changes to lectin binding during storage of RBCs suggest that significant changes occur to the carbohydrate structures at the RBC membrane. These findings provide further insight into the mechanisms of the RBC storage lesion and potential influence on RBC survival after transfusion.
膜相关碳水化合物变化作为从循环中清除衰老和受损红细胞（RBC）的信号，可能在红细胞储存损伤和输血后红细胞存活中起作用。在本研究中，一组凝集素被用于研究储存前分离为年轻和衰老红细胞的红细胞上碳水化合物的表达。
在储存前通过离心将白细胞去除的红细胞分离为年轻和衰老红细胞（n = 9对单位），并在储存42天期间的指定时间点取样。通过使用一组荧光素标记的凝集素的流式细胞术确定储存期间红细胞膜上碳水化合物表达的变化。
在整个储存过程中，衰老红细胞显示出较低的荧光强度，表明与年轻红细胞相比，凝集素的结合减少。在年轻和衰老红细胞储存期间，观察到对半乳糖和N - 乙酰葡糖胺残基具有特异性的凝集素结合逐渐增加。
红细胞储存期间凝集素结合的变化表明红细胞膜上的碳水化合物结构发生了显著变化。这些发现为红细胞储存损伤的机制以及对输血后红细胞存活的潜在影响提供了进一步的见解。
