常规储存后AS-5红细胞上清液的代谢组学
Metabolomics of AS-5 RBC supernatants following routine storage.
作者信息
D'Alessandro A, Hansen K C, Silliman C C, Moore E E, Kelher M, Banerjee A
机构信息
Department of Biochemistry and Molecular Genetics, School of Medicine University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.
出版信息
Vox Sang. 2015 Feb;108(2):131-40. doi: 10.1111/vox.12193. Epub 2014 Sep 9.
BACKGROUND AND OBJECTIVES
The safety and efficacy of stored red blood cells (RBCs) transfusion has been long debated due to retrospective clinical evidence and laboratory results, indicating a potential correlation between increased morbidity and mortality following transfusion of RBC units stored longer than 14 days. We hypothesize that storage in Optisol additive solution-5 leads to a unique metabolomics profile in the supernatant of stored RBCs.
MATERIALS AND METHODS
Whole blood was drawn from five healthy donors, RBC units were manufactured, and prestorage leucoreduced by filtration. Samples were taken on days 1 and 42, the cells removed, and mass spectrometry-based metabolomics was performed.
RESULTS
The results confirmed the progressive impairment of RBC energy metabolism by day 42 with indirect markers of a parallel alteration of glutathione and NADPH homeostasis. Moreover, oxidized pro-inflammatory lipids accumulated by the end of storage.
CONCLUSION
The supernatants from stored RBCs may represent a burden to the transfused recipients from a metabolomics standpoint.
背景与目的
由于回顾性临床证据和实验室结果表明，输注储存超过14天的红细胞（RBC）单位后发病率和死亡率增加之间可能存在关联，因此长期以来一直存在关于储存红细胞输血安全性和有效性的争论。我们假设，储存在Optisol添加剂溶液-5中会导致储存红细胞上清液中出现独特的代谢组学特征。
材料与方法
从五名健康供体采集全血，制备红细胞单位，并通过过滤进行储存前白细胞去除。在第1天和第42天取样，去除细胞，并进行基于质谱的代谢组学分析。
结果
结果证实，到第42天时红细胞能量代谢逐渐受损，谷胱甘肽和NADPH稳态平行改变的间接标志物也出现变化。此外，储存结束时氧化的促炎脂质积累。
结论
从代谢组学角度来看，储存红细胞的上清液可能会给受血者带来负担。
Zotero 插件