Duffy Kara B, Spangler Edward L, Devan Bryan D, Guo Zhihong, Bowker Jonna L, Janas Anne M, Hagepanos Adrienne, Minor Robin K, DeCabo Rafael, Mouton Peter R, Shukitt-Hale Barbara, Joseph James A, Ingram Donald K
Laboratory of Experimental Gerontology, Intramural Research Program, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA.
Neurobiol Aging. 2008 Nov;29(11):1680-9. doi: 10.1016/j.neurobiolaging.2007.04.002. Epub 2007 May 23.
Young male Fischer-344 rats were fed a diet containing 2% blueberry (BB) extract or control diet for at least 8 weeks and then received bilateral hippocampal injections of kainic acid (KA 200 ng/0.5 microl) or phosphate buffered saline (PBS). One week later rats were trained in one-way active footshock avoidance in a straight runway followed the next day by training in a footshock motivated 14-unit T-maze with documented sensitivity to hippocampal glutamatergic manipulations. Based on analyses of several performance variables, KA-treated rats exhibited clearly impaired learning performance; however, the BB diet significantly reduced this impairment. Supporting the behavioral findings, stereological assessment of CA1 pyramidal neurons documented greater neuronal loss in KA-treated controls compared to KA-treated rats on the BB diet. In an in vitro experiment, FaO cells grown in medium supplemented with serum from BB-fed rats had enhanced viability after exposure to hydrogen peroxide. These findings suggest that BB supplementation may protect against neurodegeneration and cognitive impairment mediated by excitotoxicity and oxidative stress.
将年轻雄性Fischer-344大鼠喂食含有2%蓝莓（BB）提取物的饮食或对照饮食至少8周，然后双侧海马注射海藻酸（KA 200 ng/0.5微升）或磷酸盐缓冲盐水（PBS）。一周后，大鼠在直跑道上进行单向主动电击回避训练，第二天在电击驱动的14单元T迷宫中进行训练，该迷宫对海马谷氨酸能操作具有已记录的敏感性。基于对几个行为变量的分析，KA处理的大鼠表现出明显受损的学习性能；然而，BB饮食显著降低了这种损伤。支持行为学研究结果的是，对CA1锥体神经元的立体学评估表明，与食用BB饮食的KA处理大鼠相比，KA处理的对照大鼠神经元损失更多。在一项体外实验中，在补充了来自食用BB大鼠血清的培养基中生长的FaO细胞在暴露于过氧化氢后活力增强。这些发现表明，补充BB可能预防由兴奋性毒性和氧化应激介导的神经退行性变和认知障碍。
