Naderali Elahe, Nikbakht Farnaz, Ofogh Sattar Norouzi, Rasoolijazi Homa
Anatomy Department, Medical school, Iran University of Medical Sciences, Tehran, Iran.
Physiology Department, Medical School, Iran University of Medical Sciences, Tehran, Iran.
J Integr Neurosci. 2018;17(1):19-25. doi: 10.31083/JIN-170035.
Systemic Kainic Acid (KA) administration has been used to induce experimental temporal lobe epilepsy in rats. The aim of this study was to evaluate the neuroprotective effect of rosemary extract (RE, 40% Carnosic acid) against KA-induced neurotoxicity in hippocampus and impaired learning and memory. Animals received a single dose of KA (9.5 mg/kg) intraperitoneally (i.p.) (KA group) and were observed for 2 h and were scored from 0 (for normal, no convulsion) to 5 (for continuous generalized limbic seizures). RE (100 mg/kg, orally) was administered daily for 23 days, starting a week before KA injection (KA+RE group). Neuronal degeneration in hippocampus was demonstrated by using Fluoro-Jade B immunofluorescence. The number of pyramidal cells in hippocampus was evaluated by Nissl staining. Also, the Morris Water Maze and Shuttle box have been used to assess spatial memory and passive avoidance learning, respectively. Our results revealed that, after treatment with RE, neuronal loss in CA1 decreased significantly in the animals in KA+RE group. The Morris water navigation task results revealed that spatial memory impairment decreased in the animals in KA+RE group. Furthermore, results in Shuttle box test showed that passive avoidance learning impairment significantly, upgraded in the animals in KA+RE group. These results suggest that RE may improve the spatial and working memory deficits and also neuronal degeneration induced by toxicity of KA in the rat hippocampus, due to its antioxidant activities.
全身注射海藻酸（KA）已被用于诱导大鼠实验性颞叶癫痫。本研究的目的是评估迷迭香提取物（RE，40%鼠尾草酸）对KA诱导的海马神经毒性以及学习和记忆障碍的神经保护作用。动物腹腔注射（i.p.）单剂量的KA（9.5 mg/kg）（KA组），观察2小时，并从0分（正常，无惊厥）到5分（持续全身性边缘性癫痫发作）进行评分。从KA注射前一周开始，每天口服RE（100 mg/kg），持续23天（KA+RE组）。使用Fluoro-Jade B免疫荧光法显示海马中的神经元变性。通过尼氏染色评估海马中锥体细胞的数量。此外，Morris水迷宫和穿梭箱分别用于评估空间记忆和被动回避学习。我们的结果显示，KA+RE组动物经RE治疗后，CA1区的神经元损失显著减少。Morris水迷宫任务结果显示，KA+RE组动物的空间记忆障碍有所减轻。此外，穿梭箱试验结果表明，KA+RE组动物的被动回避学习障碍显著改善。这些结果表明，由于其抗氧化活性，RE可能改善大鼠海马中由KA毒性诱导的空间和工作记忆缺陷以及神经元变性。
