Cruz Simone C, Cruz Aline C, Oliveira Juliana M, Soares Amanda M, Gioia Carolina A C, Milagres Lucimar G
Universidade do Estado do Rio de Janeiro, Av. Professor Manoel de Abreu, 444, 3 andar, Departamento de Microbiologia, Imunologia e Parasitologia, CEP: 20550-170, Rio de Janeiro, RJ, Brazil.
Vaccine. 2007 Jun 28;25(27):5046-52. doi: 10.1016/j.vaccine.2007.04.051. Epub 2007 May 7.
There is no universal vaccine against serogroup B meningococcus (Men B). We investigated the development of spleen and bone marrow-specific IgG-secreting plasma cells (ASC) in mice immunised with the Cuban outer membrane protein (OMP) vaccine (VA-MENGOC-BC). Bone marrow was the predominant anatomical site of specific ASC and showed constant ASC levels (approximately 4%) at each time point analysed, indicating the production of long-lived ASC. A mean of 2.36 and 0.35% of Men B ASC was detected in spleen after the third dose and 2 months later, respectively, indicating a short-lived population. The data suggest that a short-lived ASC population in spleen was responsible for serum IgG anti-OMP while ASC from bone marrow produced persistent bactericidal antibodies against the vaccine strain. The response to the booster dose was consistent with development of memory B cells by primary vaccination.
目前尚无针对B群脑膜炎球菌（Men B）的通用疫苗。我们研究了用古巴外膜蛋白（OMP）疫苗（VA-MENGOC-BC）免疫的小鼠中脾脏和骨髓特异性分泌IgG的浆细胞（ASC）的发育情况。骨髓是特异性ASC的主要解剖部位，在每个分析时间点均显示出稳定的ASC水平（约4%），表明产生了长寿ASC。在第三剂疫苗接种后及2个月后，脾脏中检测到的Men B ASC平均分别为2.36%和0.35%，表明这是一个短寿群体。数据表明，脾脏中的短寿ASC群体负责血清IgG抗OMP，而骨髓中的ASC产生针对疫苗菌株的持久性杀菌抗体。对加强剂量的反应与初次接种疫苗后记忆B细胞的发育一致。
