Universidade do Estado do Rio de Janeiro, Av. Professor Manoel de Abreu, 444, Departamento de Microbiologia, Imunologia e Parasitologia, CEP: 20550-170, Rio de Janeiro, RJ, Brazil.
Vaccine. 2010 Oct 4;28(42):6841-6. doi: 10.1016/j.vaccine.2010.08.036. Epub 2010 Aug 15.
Since genome sequence data became available there has been a marked increase in number of protein antigens that have been suggested as prospective vaccine components against Neisseria meningitidis B (MenB). Few studies have addressed the mechanisms by which meningococcal vaccines generate and sustain immunological memory. The goal of this study was to compare the B-cell response (antibody-secreting cells [ASC], memory B cell and IgG) evoked by a MenB vaccine (VA-MENGOC-BC(®)) with the B-cell response to diphtheria toxoid (DT) induced by a successful vaccine (Diphtheria-Tetanus-Pertussis [DTP]). The results showed different kinetics of specific ASC response after the primary and booster immunisations. Concerning the specific ASC kinetics, MenB vaccine induced a strong primary response, but the recall response showed a limited power over time. In contrast, DTP primary ASC response was weaker than the booster responses. We observed an increase in the relative percent of memory B cells after 1, 2 and 3 doses of MenB vaccine (mean of 0.8%, 1.3% and 1.6%, respectively) but without statistical significance. Similar frequencies were detected after boosting given at 4 months (mean of 1.3%) or 6 months (mean of 0.9%) following the third dose. DT specific memory B cell response showed a slight lower magnitude after the primary immunisation schedule (mean of 1.2% after the third dose) compared with the MenB response. However, a stronger memory B cell response was induced by booster doses of DTP vaccine at 4 months (mean of 1.9%) or 6 months (mean of 1.9%). The kinetics of specific IgG induced by both vaccines was similar, suggesting that memory B cells were responsible for the strong antibody response seen after the booster vaccination.
自从基因组序列数据可用以来，人们已经发现了大量的蛋白质抗原，这些抗原被认为是针对脑膜炎奈瑟菌 B 型（MenB）的潜在疫苗成分。很少有研究探讨脑膜炎球菌疫苗产生和维持免疫记忆的机制。本研究的目的是比较 MenB 疫苗（VA-MENGOC-BC(®)）引起的 B 细胞反应（抗体分泌细胞 [ASC]、记忆 B 细胞和 IgG）与成功疫苗（白喉破伤风无细胞百日咳 [DTP]）引起的 B 细胞反应。结果显示，初次免疫和加强免疫后，特异性 ASC 反应的动力学不同。关于特异性 ASC 动力学，MenB 疫苗诱导了强烈的初次反应，但随着时间的推移，回忆反应的力量有限。相比之下，DTP 的初次 ASC 反应比加强反应弱。我们观察到，在接种 MenB 疫苗 1、2 和 3 剂后，记忆 B 细胞的相对百分比增加（分别为 0.8%、1.3%和 1.6%），但无统计学意义。在第 3 剂后 4 个月（第 3 剂后平均 1.3%）或 6 个月（第 3 剂后平均 0.9%）进行加强免疫后，也检测到相似的频率。DT 特异性记忆 B 细胞反应在初次免疫方案后（第 3 剂后平均 1.2%）的幅度略低于 MenB 反应。然而，DTP 疫苗的加强剂量在 4 个月（第 3 剂后平均 1.9%）或 6 个月（第 3 剂后平均 1.9%）时可诱导更强的记忆 B 细胞反应。两种疫苗诱导的特异性 IgG 动力学相似，表明记忆 B 细胞是加强免疫后产生强烈抗体反应的原因。
