Meijssen M A, Heineman E, de Bruin R W, Veeze H J, Bijman J, de Jonge H R, ten Kate F J, Marquet R L, Molenaar J C
Department of Paediatric Surgery, Erasmus University Hospital, Rotterdam, The Netherlands.
Gut. 1991 Nov;32(11):1329-35. doi: 10.1136/gut.32.11.1329.
This study aimed to develop a non-invasive method for in vivo measurement of the transepithelial potential difference in the canine small bowel and to evaluate this parameter in small bowel autotransplants. In group 0 (control group, n = 4), two intestinal loops were created without disturbing their vascular, neural, and lymphatic supplies. In group I (successful autotransplants, n = 11), two heterotopic small bowel loops were constructed. Long term functional sequelae of vascular, neural, and lymphatic division were studied. Group II (n = 6) consisted of dogs with unsuccessful autotransplants suffering thrombosis of the vascular anastomosis, which resulted in ischaemic small bowel autografts. In group I, values of spontaneous transepithelial potential difference, an index of base line active electrolyte transport, were significantly lower compared with group 0 (p less than 0.05), probably as a result of denervation of the autotransplants. Both theophylline and glucose stimulated potential difference responses, measuring cyclic adenosine monophosphate mediated chloride secretion and sodium coupled glucose absorption respectively, showed negative luminal values in group I at all time points after transplantation. These transepithelial potential difference responses diminished progressively with time. From day 21 onwards both theophylline and glucose stimulated potential difference responses were significantly less than the corresponding responses at day seven (p less than 0.05). Morphometric analysis showed that the reduction of transepithelial potential difference responses preceded degenerative mucosal changes in the heterotopic small bowel autografts. In group II, potential difference responses to theophylline and glucose showed positive luminal values (p<0.01 v group I), probably as a result of passive potassium effusion from necrotic enterocytes.
本研究旨在开发一种用于体内测量犬小肠跨上皮电位差的非侵入性方法，并评估小肠自体移植中的该参数。在第0组（对照组，n = 4）中，创建了两个肠袢，未干扰其血管、神经和淋巴供应。在第I组（成功自体移植组，n = 11）中，构建了两个异位小肠袢。研究了血管、神经和淋巴切断的长期功能后遗症。第II组（n = 6）由自体移植不成功且血管吻合处发生血栓形成导致小肠自体移植缺血的犬组成。在第I组中，作为基线主动电解质转运指标的自发跨上皮电位差值与第0组相比显著降低（p小于0.05），这可能是自体移植去神经支配的结果。茶碱和葡萄糖刺激的电位差反应，分别测量环磷酸腺苷介导的氯离子分泌和钠偶联葡萄糖吸收，在移植后的所有时间点，第I组的管腔值均为负。这些跨上皮电位差反应随时间逐渐减弱。从第21天起，茶碱和葡萄糖刺激的电位差反应均显著低于第7天的相应反应（p小于0.05）。形态计量学分析表明，异位小肠自体移植中跨上皮电位差反应的降低先于黏膜退行性改变。在第II组中，对茶碱和葡萄糖的电位差反应显示管腔值为正（与第I组相比p<0.01），这可能是坏死肠上皮细胞被动钾外流的结果。
