Cohen Z, Nordgren S, Lossing A, Cullen J, Craddock G, Langer B
Dis Colon Rectum. 1984 Apr;27(4):228-34. doi: 10.1007/BF02553789.
The usefulness of isolated intestinal pouches of an intestinal allograft for monitoring mucosal histology during rejection episodes was studied in a canine model. Total small intestinal autotransplantation was performed in four dogs, and allotransplantation in 18 dogs. Isolated pouches from the proximal and distal ends of the allografts were brought to the skin as stomas. Serial biopsies were obtained from these pouches. Nine allotransplants were treated with a suboptimal dose of cyclosporine. Nine allografted dogs were not immunosuppressed. Biopsies from all animals were normal the first two days postoperatively, and remained so in the autotransplants. Mononuclear cell infiltration in the lamina propria and submucosa was seen up to five days before death from rejection of the allograft in dogs on cyclosporine. At autopsy, all dogs had histologic findings in the transplanted bowel similar to those of simultaneously obtained pouch biopsies. The results show that histologic changes in isolated pouches from intestinal allotransplants reflect changes in the incontinuity segment of the graft and, therefore, such pouches can be used for histologic monitoring of the graft.
在犬类模型中研究了同种异体小肠移植的孤立肠袋在排斥反应期间监测黏膜组织学的作用。对4只犬进行了全小肠自体移植，对18只犬进行了同种异体移植。将同种异体移植物近端和远端的孤立肠袋作为造口引至皮肤。从这些肠袋获取系列活检样本。9例同种异体移植用次优剂量的环孢素治疗。9只接受同种异体移植的犬未进行免疫抑制。所有动物术后前两天的活检均正常，自体移植的动物仍保持正常。在接受环孢素治疗的犬中，同种异体移植物排斥导致死亡前5天，可见固有层和黏膜下层有单核细胞浸润。尸检时，所有犬移植肠的组织学表现与同时获取的肠袋活检结果相似。结果表明，同种异体小肠移植孤立肠袋的组织学变化反映了移植物非连续性节段的变化，因此，此类肠袋可用于移植物的组织学监测。
