Paques Michel, Guyomard Jean-Laurent, Simonutti Manuel, Roux Michel J, Picaud Serge, Legargasson Jean-François, Sahel José-Alain
Department of Ophthalmology, Université Pierre et Marie Curie-Paris 6, Paris, France.
Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2769-74. doi: 10.1167/iovs.06-1099.
To analyze high-resolution color photographs of the mouse fundus.
A contact fundus camera based on topical endoscopy fundus imaging (TEFI) was built. Fundus photographs of C57 and Balb/c mice obtained by TEFI were qualitatively analyzed.
High-resolution digital imaging of the fundus, including the ciliary body, was routinely obtained. The reflectance and contrast of retinal vessels varied significantly with the amount of incident and reflected light and, thus, with the degree of fundus pigmentation. The combination of chromatic and spherical aberration favored blue light imaging, in term of both field and contrast.
TEFI is a small, low-cost system that allows high-resolution color fundus imaging and fluorescein angiography in conscious mice. Panretinal imaging is facilitated by the presence of the large rounded lens. TEFI significantly improves the quality of in vivo photography of retina and ciliary process of mice. Resolution is, however, affected by chromatic aberration, and should be improved by monochromatic imaging.
分析小鼠眼底的高分辨率彩色照片。
构建了基于局部内镜眼底成像（TEFI）的接触式眼底相机。对通过TEFI获得的C57和Balb/c小鼠的眼底照片进行定性分析。
常规获得包括睫状体在内的眼底高分辨率数字图像。视网膜血管的反射率和对比度随入射光和反射光的量而显著变化，因此也随眼底色素沉着程度而变化。就视野和对比度而言，色差和球差的组合有利于蓝光成像。
TEFI是一种小型、低成本的系统，可在清醒小鼠中进行高分辨率彩色眼底成像和荧光素血管造影。大的圆形晶状体有助于全视网膜成像。TEFI显著提高了小鼠视网膜和睫状体的体内摄影质量。然而，分辨率受色差影响，应通过单色成像加以改善。
