Taubert H, Würl P, Greither T, Kappler M, Bache M, Bartel F, Kehlen A, Lautenschläger C, Harris L C, Kaushal D, Füssel S, Meye A, Böhnke A, Schmidt H, Holzhausen H-J, Hauptmann S
Institute of Pathology, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
Oncogene. 2007 Nov 1;26(50):7170-4. doi: 10.1038/sj.onc.1210530. Epub 2007 May 21.
Cancer stem cells can play an important role in tumorigenesis and tumor progression. However, it is still difficult to detect and isolate cancer stem cells. An alternative approach is to analyse stem cell-associated gene expression. We investigated the coexpression of three stem cell-associated genes, Hiwi, hTERT and survivin, by quantitative real-time-PCR in 104 primary soft-tissue sarcomas (STS). Multivariate Cox's proportional hazards regression analyses allowed correlating gene expression with overall survival for STS patients. Coexpression of all three stem cell-associated genes resulted in a significantly increased risk of tumor-related death. Importantly, tumors of patients with the poorest prognosis were of all four tumor stages, suggesting that their risk is based upon coexpression of stem cell-associated genes rather than on tumor stage.
癌症干细胞在肿瘤发生和肿瘤进展中可发挥重要作用。然而，检测和分离癌症干细胞仍然困难。一种替代方法是分析与干细胞相关的基因表达。我们通过定量实时聚合酶链反应在104例原发性软组织肉瘤（STS）中研究了三个与干细胞相关的基因Hiwi、hTERT和生存素的共表达情况。多变量Cox比例风险回归分析使我们能够将基因表达与STS患者的总生存率相关联。所有三个与干细胞相关的基因共表达导致肿瘤相关死亡风险显著增加。重要的是，预后最差的患者的肿瘤处于所有四个肿瘤分期，这表明他们的风险基于与干细胞相关的基因共表达而非肿瘤分期。
