Xu Changqing, Qi Suxia, Gao Lei, Cui Hong, Liu Meiqiang, Yang Hongli, Li Kun, Cao Bangwei
Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong Province, China.
J Clin Immunol. 2007 Sep;27(5):534-41. doi: 10.1007/s10875-007-9095-x. Epub 2007 May 25.
Despite the availability of effective vaccines, hepatitis B virus (HBV) infection is still commonly seen worldwide. Several reports show that the human major histocompatibility complex (MHC) systems were involved in the elimination of HBV via the restrictive antigen-processing pathway. We investigate whether LMP/TAP gene polymorphisms coded by MHC-II region were associated with HBV infection. A total of seven polymorphisms of LMP/TAP gene were identified by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assays. Three hundred fifty-six patients and 326 unrelated healthy volunteers were included in the case-control study. Of the seven polymorphisms, three of which (LMP7 codons 145, TAP1 codons 637, and TAP2 codons 651) were observed to have statistically significant association with HBV infection (P < 0.05). We analyzed the three-locus haplotype constructed with three such polymorphisms and found that the frequency of haplotypes D and E increased significantly in patients, in comparison with that in controls (OR = 3.57, 95% CI: 2.09-6.12, P < 0.001; OR = 2.74, 95% CI: 1.35-5.56, P = 0.005, respectively). The results imply that LMP7-145, TAP1-637, and TAP2-651 sites were associated with the risk of HBV infection. Haplotypes D and E might be involved in the development of HBV infection. These data suggest a potential role of LMP/TAP gene as a candidate gene for susceptibility to HBV infection.
尽管有有效的疫苗，但乙型肝炎病毒（HBV）感染在全球范围内仍很常见。多项报告显示，人类主要组织相容性复合体（MHC）系统通过限制性抗原加工途径参与了HBV的清除。我们研究了MHC-II区域编码的LMP/TAP基因多态性是否与HBV感染相关。通过聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）分析共鉴定出LMP/TAP基因的7种多态性。356例患者和326名无关健康志愿者纳入病例对照研究。在这7种多态性中，观察到其中3种（LMP7第145密码子、TAP1第637密码子和TAP2第651密码子）与HBV感染有统计学显著关联（P<0.05）。我们分析了由这3种多态性构建的三位点单倍型，发现与对照组相比，患者中单倍型D和E的频率显著增加（OR=3.57，95%CI：2.09-6.12，P<0.001；OR=2.74，95%CI：1.35-5.56，P=0.005）。结果表明，LMP7-145、TAP1-637和TAP2-651位点与HBV感染风险相关。单倍型D和E可能参与了HBV感染的发生发展。这些数据表明LMP/TAP基因作为HBV感染易感性候选基因具有潜在作用。
