Khattab Ayman, Chia Yu-Shan, May Jürgen, Le Hesran Jean-Yves, Deloron Philippe, Klinkert Mo-Quen
Department for Molecular Medicine, Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Germany.
Parasitol Res. 2007 Aug;101(3):767-74. doi: 10.1007/s00436-007-0548-1. Epub 2007 May 23.
Different domains of a novel full-length var gene (termed 732var) isolated from a placenta of a malaria-infected woman were expressed in Escherichia coli as recombinant proteins and analysed biochemically and immunologically. Two of these, the Duffy binding-like (DBL)-3gamma domain and the cysteine-rich interdomain region (CIDR)-1alpha were able to bind chondroitin sulfate A and CD36, respectively. The DBL-3gamma domain was investigated in a previous study and confirmed here to exhibit anti-disease characteristics related to pregnancy-associated malaria. Mothers with high anti-DBL-3gamma antibody levels were protected from placental infection. The novel finding in this study is that babies born to mothers carrying anti-CIDR-1alpha antibodies had a delayed time to the first infection.
从一名感染疟疾妇女的胎盘中分离出的一种新型全长var基因（称为732var）的不同结构域，在大肠杆菌中表达为重组蛋白，并进行了生化和免疫学分析。其中两个结构域，即达菲结合样（DBL）-3γ结构域和富含半胱氨酸的结构域间区域（CIDR）-1α，分别能够结合硫酸软骨素A和CD36。DBL-3γ结构域在先前的一项研究中已被研究，在此得到证实，其具有与妊娠相关疟疾相关的抗病特性。抗DBL-3γ抗体水平高的母亲可免受胎盘感染。本研究中的新发现是，携带抗CIDR-1α抗体的母亲所生的婴儿首次感染的时间延迟。
