Christensen Jeppe Romme, Larsen Karen Bonde, Lisanby Sarah H, Scalia Jason, Arango Victoria, Dwork Andrew J, Pakkenberg Bente
Research Laboratory for Stereology and Neuroscience, Bispebjerg University Hospital, Copenhagen, Denmark.
Anat Rec (Hoboken). 2007 Mar;290(3):330-40. doi: 10.1002/ar.20504.
The rhesus monkey is widely used as an experimental animal model in the study of brain function and disease. While previous quantitative studies have provided knowledge of regional numbers, little is known of the total neocortical neuron and glial cell numbers in this species. The aim of this study is to establish quantitative norms. We use the optical fractionator and Cavalieri principle to examine the right hemisphere of eight young rhesus monkeys taken from the control group of an ongoing study. Applying these methods to agar-embedded and vibratome-sectioned tissue, we generate estimates of cell numbers and regional volumes of neocortical and hippocampal regions with coefficients of variance (CV) around 10%. The mean unilateral neocortical neuron number is 1.35 x 10(9) (CV +/- 0.10) and the mean unilateral neocortical glial cell number is 0.78 x 10(9) (CV +/- 0.17). Mean unilateral neocortical volume is found to be 8.5 (CV +/- 0.10) cm(3) after processing, or 19 cm(3) when correcting for shrinkage. The neuron/glia ratio is 1.77. The neurons are distributed with 18% in the frontal cortex, 57% in the temporal and parietal cortices, and 25% in the occipital cortex. In the hippocampal subregions, we found unilateral neuron number of 1.72 x 10(6) (CV +/- 0.13) and glial number of 2.25 x 10(6) (CV +/- 0.17) in CA1, and 0.80 x 10(6) (CV +/- 0.27) neurons and 1.05 x 10(6) (CV +/- 0.26) glial cells in CA2-3. Comparisons with related studies show quantitative variation, but also variations in methods and applications. The results are phylogenetically consistent, apart from the neuron/glia ratio, which is remarkably higher than what is found in other species.
恒河猴被广泛用作脑功能和疾病研究的实验动物模型。虽然先前的定量研究提供了区域数量方面的知识，但对于该物种新皮层神经元和神经胶质细胞的总数知之甚少。本研究的目的是建立定量标准。我们使用光学分割器和卡瓦列里原理对来自一项正在进行的研究对照组的八只幼年恒河猴的右半球进行检查。将这些方法应用于琼脂包埋和振动切片组织，我们得出新皮层和海马区细胞数量及区域体积的估计值，变异系数（CV）约为10%。单侧新皮层神经元的平均数量为1.35×10⁹（CV±0.10），单侧新皮层神经胶质细胞的平均数量为0.78×10⁹（CV±0.17）。处理后单侧新皮层的平均体积为8.5（CV±0.10）cm³，校正收缩后为19 cm³。神经元与神经胶质细胞的比例为1.77。神经元分布在额叶皮层的占18%，颞叶和顶叶皮层的占57%，枕叶皮层的占25%。在海马亚区，我们发现CA1区单侧神经元数量为1.72×10⁶（CV±0.13），神经胶质细胞数量为2.25×10⁶（CV±0.17），CA2 - 3区有0.80×10⁶（CV±0.27）个神经元和1.
