Desai Varsha G, Lee Taewon, Delongchamp Robert R, Moland Carrie L, Branham William S, Fuscoe James C, Leakey Julian E A
Center for Functional Genomics, Division of Systems Toxicology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA.
Mitochondrion. 2007 Sep;7(5):322-9. doi: 10.1016/j.mito.2007.02.004. Epub 2007 Mar 6.
This study describes the development of a mitochondria-specific microarray, MitoChip, to measure transcripts of mitochondria-associated genes in various diseases and drug-induced toxicities in the mouse. The array consists of 542 oligonucleotides that represent genes from the mitochondrial and nuclear genomes associated with mitochondrial structure and functions. The expression of mitochondrial genes was measured in the liver of both p53 haplodeficient (+/-) and wild-type (+/+) C3B6F(1) female mice exposed to antiretroviral agents, Zidovudine (AZT) and Lamivudine (3TC). Among genes whose expression was significantly altered, a set was selected for real-time PCR analysis to verify their differential gene expression. The real-time PCR data confirmed the observations by microarray analysis suggesting that the MitoChip may be an important tool for examining mitochondrial involvement in diseases and drug-induced toxicities.
本研究描述了一种线粒体特异性微阵列芯片（MitoChip）的开发，用于测量小鼠各种疾病和药物诱导毒性中线粒体相关基因的转录本。该阵列由542个寡核苷酸组成，这些寡核苷酸代表与线粒体结构和功能相关的线粒体和核基因组中的基因。在暴露于抗逆转录病毒药物齐多夫定（AZT）和拉米夫定（3TC）的p53单倍体缺陷（+/-）和野生型（+/+）C3B6F(1)雌性小鼠的肝脏中测量线粒体基因的表达。在表达显著改变的基因中，选择一组进行实时PCR分析以验证其差异基因表达。实时PCR数据证实了微阵列分析的观察结果，表明MitoChip可能是检查线粒体在疾病和药物诱导毒性中所起作用的重要工具。
