Stephanidis B, Adichtchev S, Gouet P, McPherson A, Mermet A
Laboratoire de Physico-Chimie des Matériaux Luminescents, Université Lyon 1, Villeurbanne, France.
Biophys J. 2007 Aug 15;93(4):1354-9. doi: 10.1529/biophysj.107.109033. Epub 2007 May 25.
Viruses are compact biological nanoparticles whose elastic and dynamical properties are hardly known. Inelastic (Brillouin) light scattering was used to characterize these properties, from microcrystals of the Satellite Tobacco Mosaic Virus, a nearly spherical plant virus of 17-nm diameter. Longitudinal sound velocities in wet and dry Satellite Tobacco Mosaic Virus crystals were determined and compared to that of the well-known protein crystal, lysozyme. Localized vibrational modes of the viral particles (i.e., particle modes) were sought in the relevant frequency ranges, as derived assuming the viruses as full free nanospheres. Despite very favorable conditions, regarding virus concentration and expected low damping in dry microcrystals, no firm evidence of virus particle modes could be detected.
病毒是紧凑的生物纳米颗粒，其弹性和动力学特性几乎不为人知。非弹性（布里渊）光散射被用于表征这些特性，研究对象是烟草花叶卫星病毒的微晶，这是一种直径为17纳米的近球形植物病毒。测定了湿润和干燥的烟草花叶卫星病毒晶体中的纵向声速，并与著名的蛋白质晶体溶菌酶的声速进行了比较。在相关频率范围内寻找病毒颗粒的局域振动模式（即颗粒模式），这是假设病毒为完全自由的纳米球推导出来的。尽管在病毒浓度和干燥微晶中预期的低阻尼方面条件非常有利，但未检测到病毒颗粒模式的确切证据。
