Welker Noah C, Habig Jeffrey W, Bass Brenda L
Department of Biochemistry and Howard Hughes Medical Institute, University of Utah, Salt Lake City, UT, 84112, USA.
RNA. 2007 Jul;13(7):1090-102. doi: 10.1261/rna.542107. Epub 2007 May 25.
We describe the first microarray analysis of a whole animal containing a mutation in the Dicer gene. We used adult Caenorhabditis elegans and, to distinguish among different roles of Dicer, we also performed microarray analyses of animals with mutations in rde-4 and rde-1, which are involved in silencing by siRNA, but not miRNA. Surprisingly, we find that the X chromosome is greatly enriched for genes regulated by Dicer. Comparison of all three microarray data sets indicates the majority of Dicer-regulated genes are not dependent on RDE-4 or RDE-1, including the X-linked genes. However, all three data sets are enriched in genes important for innate immunity and, specifically, show increased expression of innate immunity genes.
我们描述了对一只含有Dicer基因突变的完整动物进行的首次微阵列分析。我们使用了成年秀丽隐杆线虫，并且为了区分Dicer的不同作用，我们还对rde - 4和rde - 1发生突变的动物进行了微阵列分析，rde - 4和rde - 1参与小干扰RNA（siRNA）介导的沉默作用，但不参与微小RNA（miRNA）介导的沉默作用。令人惊讶的是，我们发现X染色体上受Dicer调控的基因高度富集。对所有三个微阵列数据集的比较表明，大多数受Dicer调控的基因不依赖于RDE - 4或RDE - 1，包括X连锁基因。然而，所有三个数据集都富含对先天免疫重要的基因，并且具体表现为先天免疫基因的表达增加。
