Port K
Department of Medical and Biological Disciplines, E. Vilde's Teachers Training Institute of Tallinn, Estonia.
Int J Sports Med. 1991 Oct;12(5):490-4. doi: 10.1055/s-2007-1024720.
In six male physically active subjects the adrenocortical and metabolic changes in response to incremental exercise testing were investigated. Blood and saliva samples were taken at rest, at the end of every workload (duration 4 min with 50 W increment), immediately and 10 min after 1 min all out spurt on the electrically braked cycle ergometer. Both saliva and serum cortisol were measured as well as blood lactate. The cortisol response in serum and in saliva showed similar dynamics (r = 0.86, p less than 0.001, n = 50) at submaximal work. At maximal work the serum cortisol concentration showed a transitory decrease, which was not manifested in saliva. It is hypothesized that a factor related to the metabolic acidosis masks the actual adrenocortical response in the serum but not in saliva. Correlation analysis revealed a positive relation between lactate and cortisol in serum (r = 0.56, p less than 0.01, n = 50) and saliva (r = 0.70, p less than 0.01, n = 50). Apparently, salivary cortisol closely reflects plasma free cortisol level, presenting advantage over total cortisol measurements. Moreover salivary measurement will permit studies in their authentic settings and should assist attempts to understand the nature of the adrenocortical function in exercise.
在六名身体活跃的男性受试者中，研究了递增运动测试后肾上腺皮质和代谢的变化。在静息状态、每个工作负荷结束时（持续4分钟，每次增加50瓦）、在电动刹车的自行车测力计上进行1分钟全力冲刺后即刻以及10分钟后采集血液和唾液样本。测量了唾液和血清皮质醇以及血乳酸。在次最大运动量时，血清和唾液中的皮质醇反应显示出相似的动态变化（r = 0.86，p < 0.001，n = 50）。在最大运动量时，血清皮质醇浓度出现短暂下降，而唾液中未出现这种情况。据推测，与代谢性酸中毒相关的一个因素掩盖了血清中实际的肾上腺皮质反应，但在唾液中未出现这种情况。相关性分析显示，血清（r = 0.56，p < 0.01，n = 50）和唾液（r = 0.70，p < 0.01，n = 50）中的乳酸与皮质醇之间呈正相关。显然，唾液皮质醇能密切反映血浆游离皮质醇水平，比总皮质醇测量具有优势。此外，唾液测量将允许在真实环境中进行研究，并应有助于理解运动中肾上腺皮质功能的本质。
