Crewther Blair T, Obmiński Zbigniew, Orysiak Joanna, Al-Dujaili Emad A S
Institute of Sport-National Research Institute, Warsaw, Poland.
Centre for Cardiovascular Sciences, Edinburgh University, Edinburgh, UK.
J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22197. Epub 2017 Mar 13.
This study examined the utility of salivary testosterone (T) and cortisol (C) concentration measures for assessing the acute stress responses of junior athletes during a sporting competition.
Paired capillary blood and saliva samples were taken from 71 junior athletes (45 males, 26 females) before and after a simulated Olympic weightlifting competition. The concentration measures of salivary T and C were referenced against blood total T (TT) and C (TC) concentrations. All hormones were tested by immunoassay.
The competition promoted an increase in the blood TT concentrations of the male (10.1%±18.7%) and female (12.9%±19.9%) athletes (P<.05), with TC also rising in females (37.5%±69%) and tending to increase in males (23.2%±56.1%). No significant changes in salivary T or C were identified. Locally-weighted regression revealed that salivary T was strongly related to blood TT in males (r =.84), but not females (r =-.15), whereas salivary C was moderately related to blood TC in both sexes (r =.43, r =.48), respectively.
A short, high-intensity competition promoted a predictable stress-induced rise in blood TT and TC concentrations. We observed no hormonal stress responses in saliva under the current format, which could be explained by individual variability and blood-saliva differences in hormone kinetics. Still, the salivary T and C measures did show a moderate to strong ability to track individual blood TT (males only) and TC (both sexes) concentrations in a young athletic cohort across a broad physiological range. Recommendations for researchers and practitioners are presented.
本研究探讨了唾液睾酮（T）和皮质醇（C）浓度测量在评估青少年运动员体育比赛期间急性应激反应中的作用。
在模拟奥运会举重比赛前后，从71名青少年运动员（45名男性，26名女性）采集配对的毛细血管血和唾液样本。唾液T和C的浓度测量以血液总T（TT）和C（TC）浓度为参照。所有激素均通过免疫测定法检测。
比赛促使男性（10.1%±18.7%）和女性（12.9%±19.9%）运动员的血液TT浓度升高（P<.05），女性的TC也升高（37.5%±69%），男性有升高趋势（23.2%±56.1%）。未发现唾液T或C有显著变化。局部加权回归显示，男性唾液T与血液TT密切相关（r =.84），而女性则不然（r = -.15），而唾液C在两性中与血液TC均呈中度相关（分别为r =.43，r =.48）。
一场短暂、高强度的比赛促使血液TT和TC浓度因应激而出现可预测的升高。在当前模式下，我们未观察到唾液中的激素应激反应，这可能由个体差异以及激素动力学中的血-唾液差异来解释。尽管如此，唾液T和C测量在一个年轻运动员队列的广泛生理范围内，确实显示出了中度至较强的追踪个体血液TT（仅男性）和TC（两性）浓度的能力。本文为研究人员和从业者提供了建议。
