储存对马、牛、羊和犬静脉血中离子钙及酸碱变量测量的影响。
Effect of storage on measurement of ionized calcium and acid-base variables in equine, bovine, ovine, and canine venous blood.
作者信息
Szenci O, Brydl E, Bajcsy C A
机构信息
Department of Obstetrics and Reproductive Biology, University of Veterinary Science, Budapest, Hungary.
出版信息
J Am Vet Med Assoc. 1991 Nov 1;199(9):1167-9.
The stability of blood ionized calcium (Ca2+) and acid-base variables in equine, bovine, ovine, and canine venous blood samples (n = 15, in each group) stored at 4 C for 3, 6, 9, 24, or 48 hours was studied. Variables included blood Ca2+ and standard ionized calcium (Ca2+ corrected to pH 7.4) concentrations, pH, blood carbon dioxide and oxygen tensions, base excess, bicarbonate concentration, and total carbon dioxide content. Results indicate that storage of blood samples at 4 C for up to 48 hours, despite appreciable acid-base changes, is associated with less than 1.5% change in equine, bovine, and ovine blood Ca2+ concentrations. Similar changes were observed in canine blood during the first 9 hours' storage. After 24 and 48 hours' storage, clinically relevant decrease (10.5 and 15.5%) in canine blood Ca2+ concentration was measured. Therefore, Ca2+ concentration in equine, bovine, and ovine venous blood samples stored up to 48 hours, and in canine blood samples stored up to 9 hours at 4 C is of diagnostic use.
研究了马、牛、羊和犬的静脉血样本（每组n = 15）在4℃下储存3、6、9、24或48小时后血液中离子钙（Ca2+）的稳定性以及酸碱变量。变量包括血液Ca2+浓度、标准离子钙（校正至pH 7.4的Ca2+）浓度、pH值、血液二氧化碳和氧气分压、碱剩余、碳酸氢盐浓度以及总二氧化碳含量。结果表明，在4℃下将血样储存长达48小时，尽管酸碱发生了明显变化，但马、牛和羊血液中Ca2+浓度的变化小于1.5%。在犬血储存的前9小时内观察到类似变化。在储存24和48小时后，测量到犬血中Ca2+浓度出现了临床上相关的降低（分别为10.5%和15.5%）。因此，在4℃下储存长达48小时的马、牛和羊静脉血样本以及储存长达9小时的犬血样本中的Ca2+浓度具有诊断价值。
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