Curtis P, Amso N, Keith E, Bernard A, Shaw R W
Academic Department of Obstetrics and Gynaecology, Royal Free Hospital, Hampstead, London, UK.
Hum Reprod. 1991 Oct;6(9):1294-7. doi: 10.1093/oxfordjournals.humrep.a137530.
The risk of introducing infection into the peritoneal cavity at the time of transvaginal ultrasound-guided recovery of oocytes in assisted reproduction techniques was assessed by culturing peritoneal fluid samples from 25 women with unexplained infertility. The samples were collected laparoscopically at the time of zygote intra-Fallopian transfer (ZIFT), 24-48 h after oocyte collection. High vaginal and endocervical specimens from 25 women treated by in-vitro fertilization (IVF) and transcervical embryo transfer were cultured for comparison. The peritoneal cultures were negative in all but one patient. High vaginal swabs grew Candida albicans in three cases and endocervical specimens were all negative. Seven and three pregnancies occurred in the ZIFT and IVF groups respectively. No pelvic damage was noted at laparoscopy in those women who had had previous treatment cycles with transvaginal oocyte recovery. This method of oocyte recovery, using prophylactic metronidazole and chlorhexidine for preoperative vaginal preparation, appears to be safe for treatment of women with no previous pelvic damage.
通过对25例不明原因不孕症女性的腹腔液样本进行培养，评估了在辅助生殖技术中经阴道超声引导取卵时将感染引入腹腔的风险。样本在受精卵输卵管内移植（ZIFT）时通过腹腔镜采集，即在取卵后24 - 48小时。对25例接受体外受精（IVF）和经宫颈胚胎移植治疗的女性的高阴道和宫颈管标本进行培养以作比较。除1例患者外，所有腹腔培养均为阴性。高阴道拭子在3例中培养出白色念珠菌，宫颈管标本均为阴性。ZIFT组和IVF组分别有7例和3例妊娠。在那些先前有过经阴道取卵治疗周期的女性中，腹腔镜检查未发现盆腔损伤。这种使用预防性甲硝唑和洗必泰进行术前阴道准备的取卵方法，对于既往无盆腔损伤的女性治疗似乎是安全的。
