Knutsson Johan, Bagger-Sjöbäck Dan, von Unge Magnus
Center for Hearing and Communication Research, Karolinska Institute, Stockholm, Sweden.
Otol Neurotol. 2007 Jun;28(4):486-91. doi: 10.1097/01.mao.0000265202.85119.1c.
The objective of the study was to investigate the histological distribution of collagens in the healthy rat's tympanic membrane.
Immunohistochemical analysis of collagen type I, II, III, and IV in the tympanic membranes in healthy adult female Sprague-Dawley rats. The staining was semiquantified using light microscopy in a blinded fashion, not knowing what type of collagen the slide had been stained for.
The pars tensa of the tympanic membrane was mainly stained for collagen type II and IV. The fibrous annulus could on immunohistochemistry be subdivided into an inner and an outer portion. The inner portion of the fibrous annulus was mainly stained for collagen type II, whereas the outer portion was most strongly stained for collagen type III and collagen type IV. The test-retest reliability of the semiquantative method was 81%.
Collagen type II and IV are the major collagen constituents of the pars tensa of the tympanic membrane. The outer portion of the fibrous annulus has collagen type III and IV as its major constituents, whereas the inner portion is made up of collagen type II.
本研究的目的是调查健康大鼠鼓膜中胶原蛋白的组织学分布。
对健康成年雌性斯普拉格-道利大鼠的鼓膜进行I型、II型、III型和IV型胶原蛋白的免疫组织化学分析。使用光学显微镜以盲法对染色进行半定量分析，不知道玻片上所染的是哪种类型的胶原蛋白。
鼓膜的紧张部主要被II型和IV型胶原蛋白染色。纤维环在免疫组织化学上可分为内部和外部。纤维环的内部主要被II型胶原蛋白染色，而外部被III型和IV型胶原蛋白染色最深。半定量方法的重测信度为81%。
II型和IV型胶原蛋白是鼓膜紧张部的主要胶原蛋白成分。纤维环的外部以III型和IV型胶原蛋白为主要成分，而内部由II型胶原蛋白组成。
