Department of Otorhinolaryngology, Umeå University, Umeå, Sweden.
Otol Neurotol. 2013 Sep;34(7):e88-92. doi: 10.1097/MAO.0b013e3182908b51.
Type II collagen is synthesized in the tympanic membrane during healing of a perforation. The time course and appearance of Type II collagen regrowth in the newly healed tympanic membrane is the subject of the present study.
The arrangement of Type II collagen fibers in the tympanic membrane gives it a unique tensile strength important for sound conduction. Healing after tympanic membrane perforation can cause the tympanic membrane to lose its characteristic. At what phase Type II collagen in tympanic membrane is replaced during healing of a perforation has previously not been studied.
Rat tympanic membranes were perforated, and the animals were sacrificed 9 to 16 days after perforation. Tympanic membranes were stained with a Type II collagen antibody.
At Day 9, a majority of the tympanic membranes had healed. Keratinizing epithelium and connective tissue had formed, but there was no Type II collagen. At Day 10, all tympanic membranes had healed, and staining for Type II collagen appeared. After Day 10, staining was more intense. Newly formed collagen did not show the parallel bundle arrangement seen in normal tympanic membranes but was more scattered in the tissue.
Type II collagen was seen in tympanic membranes only after closure of the perforation. The fiber arrangement after healing was disturbed, which presumably has an impact on the function of the tympanic membrane. Understanding the formation of Type II collagen in the healing of tympanic membrane perforations could enable further research toward treating tympanic membrane perforations.
在鼓膜穿孔愈合过程中，II 型胶原蛋白在鼓膜中合成。本研究的主题是新愈合的鼓膜中 II 型胶原蛋白再生的时间过程和外观。
鼓膜中 II 型胶原蛋白纤维的排列赋予了它独特的拉伸强度，这对于声音传导很重要。鼓膜穿孔后的愈合可能会导致鼓膜失去其特征。在鼓膜穿孔愈合过程中，II 型胶原蛋白在何时被替代以前尚未研究过。
大鼠鼓膜穿孔，穿孔后 9 至 16 天处死动物。用 II 型胶原蛋白抗体对鼓膜进行染色。
第 9 天，大多数鼓膜已愈合。角化上皮和结缔组织已形成，但没有 II 型胶原蛋白。第 10 天，所有鼓膜均已愈合，出现 II 型胶原蛋白染色。第 10 天之后，染色更加明显。新形成的胶原蛋白没有表现出正常鼓膜中所见的平行束排列，而是在组织中更加分散。
只有在穿孔闭合后，才能在鼓膜中看到 II 型胶原蛋白。愈合后的纤维排列受到干扰，这可能对鼓膜的功能产生影响。了解鼓膜穿孔愈合过程中 II 型胶原蛋白的形成，可能有助于进一步研究治疗鼓膜穿孔的方法。
