Dai Chenkai, Wood Mark W, Gan Rong Z
University of Oklahoma, Norman, Oklahoma, USA.
Otol Neurotol. 2007 Jun;28(4):551-8. doi: 10.1097/mao.0b013e318033f008.
The aim of this study is to investigate the effect of middle ear fluid and pressure on tympanic membrane mobility by using laser Doppler interferometry and to compare these results with tympanometry.
Tympanometry has been commonly used for evaluation of otitis media with effusion, a middle ear disease with fluid in the cavity. However, this test lacks specific interpretations of middle ear disorders based on tympanometric data. Laser interferometry, as an advanced research tool to measure middle ear function, may provide knowledge of how tympanic membrane mobility is affected by middle ear fluid and pressure.
An otitis media with effusion model was created in seven human temporal bones for conducting experiments with tympanometry and laser interferometry. Middle ear pressure varied from -20 to +20 cm water, and the amount of fluid in the middle ear was gradually increased to fill the cavity.
The displacement of the tympanic membrane measured by laser interferometry at selected frequencies decreased significantly corresponding to the middle ear air pressure changes. Tympanometry detected middle ear pressure by the change of tympanometric peak location, but the tympanogram shape was not affected by the middle ear pressure. The middle ear fluid was detected by tympanometry with as little as 0.3 mL, and laser interferometry was able to measure the displacement change of the tympanic membrane with 0.2 or 0.3 mL fluid at different frequencies.
Laser interferometry can detect the effect of middle ear pressure and fluid on tympanic membrane movement as well as tympanometry does.
本研究旨在通过激光多普勒干涉测量法研究中耳积液和压力对鼓膜活动度的影响，并将这些结果与鼓室导抗图测量结果进行比较。
鼓室导抗图测量法一直常用于评估中耳积液性中耳炎，即中耳腔有积液的一种中耳疾病。然而，该测试缺乏基于鼓室导抗图数据对中耳疾病的具体解释。激光干涉测量法作为一种测量中耳功能的先进研究工具，可能会提供有关鼓膜活动度如何受中耳积液和压力影响的知识。
在七块人类颞骨上建立中耳积液模型，用于进行鼓室导抗图测量和激光干涉测量实验。中耳压力在-20至+20厘米水柱之间变化，中耳内的液体量逐渐增加以充满中耳腔。
通过激光干涉测量法在选定频率下测量的鼓膜位移随中耳气压变化而显著降低。鼓室导抗图测量法通过鼓室导抗图峰值位置的变化检测中耳压力，但鼓室导抗图形状不受中耳压力影响。鼓室导抗图测量法能检测到低至0.3毫升的中耳积液，激光干涉测量法能够在不同频率下测量0.2或0.3毫升液体时鼓膜的位移变化。
激光干涉测量法在检测中耳压力和积液对鼓膜运动的影响方面与鼓室导抗图测量法一样有效。
