Hussein Mohamed Hamed, Daoud Ghada A, Kakita Hiroki, Hattori Ayako, Murai Hiroki, Yasuda Mari, Mizuno Keisuke, Goto Kenji, Ozaki Yasuhiko, Ito Tetsuya, Tanaka Taihei, Fukuda Sumio, Kato Ineko, Fujimoto Shinji, Suzuki Satoshi, Sobajima Hisanori, Togari Hajime
Department of Pediatrics, Neonatology and Congenital Disorders, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Misuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Shock. 2007 Aug;28(2):154-9. doi: 10.1097/shk.0b013e31803dcf55.
Newborn males are more sensitive to brain injury than newborn females are. The aim of the present study was to find an explanation for this. We used the neuron-specific enolase (NSE) levels in the cerebrospinal fluid (CSF) for the classification of 32 newborns (19 males and 13 females) on their fifth postnatal day. The NSE levels were higher than normal (8.4 +/- 1.6 ng/mL) in 10 newborn males and 6 females and were, respectively, considered asphyxiated male and female groups. The remaining newborns, 9 males and 7 females, had normal CSF levels of NSE and were considered normal newborn male and female groups. The CSF samples were measured for 12 cytokines, using a cytokine array kit, and for total hydroperoxide and biological antioxidant potentials (BAPs), using the free radical analytic system. Among the 12 cytokines measured, only interleukin 8 (IL-8) was properly detected. The CSF levels of IL-8 were higher in the asphyxiated newborn females than in the other three groups. The mean CSF levels of BAPs in the asphyxiated newborn females were higher compared with the other three groups, but significance was detected only in comparison with the BAP levels in the CSF samples of the normal newborn males. There were no differences in total hydroperoxide levels among the groups. There are sex-related differences in the CSF levels of IL-8 and antioxidants in asphyxiated newborns, with higher levels in newborn females; this might contribute in the sexual dimorphism regarding the fact that females have better protection from brain injury than the males.
新生男婴比新生女婴对脑损伤更敏感。本研究的目的是找到对此现象的一种解释。我们利用出生后第5天32名新生儿（19名男婴和13名女婴）脑脊液中的神经元特异性烯醇化酶（NSE）水平进行分类。10名男婴和6名女婴的NSE水平高于正常水平（8.4±1.6 ng/mL），分别被视为窒息男婴组和窒息女婴组。其余新生儿，9名男婴和7名女婴，脑脊液NSE水平正常，被视为正常男婴组和正常女婴组。使用细胞因子阵列试剂盒对脑脊液样本检测12种细胞因子，并使用自由基分析系统检测总氢过氧化物和生物抗氧化能力（BAP）。在所检测的12种细胞因子中，仅正确检测到白细胞介素8（IL-8）。窒息新生女婴脑脊液中IL-8水平高于其他三组。窒息新生女婴脑脊液中BAP的平均水平高于其他三组，但仅与正常新生男婴脑脊液样本中的BAP水平相比有显著性差异。各组间总氢过氧化物水平无差异。窒息新生儿脑脊液中IL-8和抗氧化剂水平存在性别差异，女婴水平较高；这可能有助于解释女性比男性对脑损伤有更好保护这一性别差异现象。
